Development of the second‐order derivative UV spectrophotometric method for direct determination of paracetamol in urine intended for biopharmaceutical characterisation of drug products
Open Access
- 14 July 2003
- journal article
- research article
- Published by Wiley in Biopharmaceutics & Drug Disposition
- Vol. 24 (7), 309-314
- https://doi.org/10.1002/bdd.367
Abstract
Paracetamol is a widely used nonsalicylate analgesic and antipyretic drug. The existing methods for the determination of paracetamol in biological fluids are mainly HPLC techniques, although there are some reported methods based on spectrophotometric determinations. However, all these methods involve some extraction or derivatisation procedures. In the present study the UV spectra of investigated samples were recorded over the wavelength range 220–400 nm (λ step 0.21 nm; scan speed 60 nm/min) and second-order derivative spectra were calculated. Second-order derivative spectra of different blank urine samples displayed the presence of a zero-crossing point at 245–247 nm defined as λzc. The zero-order absorption spectra of paracetamol in water displays maximum absorbance at 243 nm, while in second derivative spectra, a minimum peak at 246 nm was observed. Therefore, the application of zero-crossing technique to the second-derivative UV absorption spectrum should be useful for the determination of paracetamol using 2Dλzc. The proposed method enables determination of total paracetamol in urine directly and simply by reading the 2Dλzc of the diluted samples. The obtained results were in good accordance with published data on cumulative urinary excretion after per oral administration of paracetamol obtained applying different spectrophotometric methods of determination. It could be useful for biopharmaceutical characterisation of drug products (monitoring of the levels of paracetamol in urine in bioavailability testing, for the evaluation of in vitro–in vivo correlation and screening of different formulations during drug product development). Copyright © 2003 John Wiley & Sons, Ltd.Keywords
This publication has 14 references indexed in Scilit:
- Bioequivalence Study of Paracetamol Tablets: In Vitro-In Vivo CorrelationDrug Development and Industrial Pharmacy, 2000
- Rapid Determination of Paracetamol in Blood Serum Samples by First-Derivative UV Absorption SpectroscopyAnalytical Letters, 1995
- Spectrofluorimetric determination of paracetamol in pharmaceuticals and biological fluidsJournal of Pharmaceutical and Biomedical Analysis, 1995
- Assay of paracetamol and its metabolites in urine, plasma and saliva of children with chronic liver diseaseJournal of Pharmaceutical and Biomedical Analysis, 1995
- Comparison of an Antiinflammatory Dose of Ibuprofen, an Analgesic Dose of Ibuprofen, and Acetaminophen in the Treatment of Patients with Osteoarthritis of the KneeThe New England Journal of Medicine, 1991
- Application of Second-Derivate Spectroscopy to the Simultaneous Identification and Determination of Plasma Salicylate and ParacetamolAnalytical Letters, 1991
- The effect of the molecular weight of polyethylene glycol on the bioavailability of paracetamol-polyethylene glycol solid dispersionsJournal of Pharmacy and Pharmacology, 1986
- Comparative Bioavailability of Three Commercial Acetaminophen TabletsJournal of Pharmaceutical Sciences, 1981
- High‐Pressure Liquid Chromatographic Determination of Acetaminophen in Biological FluidsJournal of Pharmaceutical Sciences, 1976
- Acetaminophen III: Dissolution Studies of Commercial Tablets of Acetaminophen and Comparison with In Vivo Absorption ParametersJournal of Pharmaceutical Sciences, 1971