Methyllycaconitine and (+)‐anatoxin‐a differentiate between nicotinic receptors in vertebrate and invertebrate nervous systems
- 4 January 1988
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 226 (2), 357-363
- https://doi.org/10.1016/0014-5793(88)81454-6
Abstract
Specific high-affinity binding sites for 125I-alpha-bungarotoxin and (-)-[3H]nicotine have been measured in rat brain and locust (Schistocerca gregaria) ganglia. The binding sites for 125I-alpha-bungarotoxin had similar Kd values of 1.5 x 10(-9) and 0.8 x 10(-9) M for rat and locust preparations, respectively; the corresponding values for the (-)-[3H]nicotine-binding site were 9.3 x 10(-9) and 1.7 x 10(-7) M. Methyllycaconitine (MLA) potently inhibited 125I-alpha-bungarotoxin binding in both rat and locust. MLA was a less effective inhibitor of (-)-[3H]nicotine binding whereas (+)-anatoxin-a was a very potent inhibitor at this site in the rat but not in the locust. These data suggest that (+)-anatoxin-a is a useful probe for the high-affinity nicotine-binding receptor in vertebrate brain, whereas MLA is a preferential probe for the subclass of receptor that binds alpha-bungarotoxin.Keywords
This publication has 19 references indexed in Scilit:
- Nicotinic acetylcholine receptors in cultured neurons from the hippocampus and brain stem of the rat characterized by single channel recordingFEBS Letters, 1987
- Brain Nicotine Binding SitesHuman Toxicology, 1987
- Members of a nicotinic acetylcholine receptor gene family are expressed in different regions of the mammalian central nervous systemCell, 1987
- Is the insect neuronal nAChR the ancestral ACh receptor protein?Trends in Neurosciences, 1986
- α‐Bungarotoxin Binds to Low‐Affinity Nicotine Binding Sites in Rat BrainJournal of Neurochemistry, 1986
- ?-Aminobutyric Acid Receptor Complex of Insect CNS: Characterization of a Benzodiazepine Binding SiteJournal of Neurochemistry, 1986
- Methyllycaconitine, a naturally occurring insecticide with a high affinity for the insect cholinergic receptorCellular and Molecular Life Sciences, 1986
- A rapid filtration assay for soluble receptors using polyethylenimine-treated filtersAnalytical Biochemistry, 1983
- Interrelationship of carbohydrate and the α-toxin binding site on the acetylcholine receptor from torpedomarmorataLife Sciences, 1980
- The principal toxin ofDelphinium brownii Rydb., and its mode of actionCellular and Molecular Life Sciences, 1979