Stability of proposed biomarkers of prenatal androgen exposure over the menstrual cycle
- 13 January 2015
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Developmental Origins of Health and Disease
- Vol. 6 (2), 149-157
- https://doi.org/10.1017/s2040174414000646
Abstract
The prenatal hormonal milieu is widely believed to shape health later in life; however, there are considerable methodological challenges associated with measuring the in utero hormonal environment. Two potential biomarkers of prenatal androgen exposure that can be measured postnatally have been proposed: anogenital distance (AGD) and the ratio of the second to fourth digits of the hand (2D:4D). Although both measures are widely used research tools, their use in adult women may be complicated by the dramatic fluctuations in reproductive hormones across the menstrual cycle. To determine whether there is cyclical variation in these biomarkers, we conducted a longitudinal study of 12 naturally cycling, nulliparous adult women. Trained examiners assessed two measures of AGD [anus to clitoris (AGD-AC) and anus to fourchette (AGD-AF)] and 2D:4D in both hands for the duration of three menstrual cycles, taking measurements during the follicular, peri-ovulatory and luteal phases of each cycle. Despite the small sample size, longer (more masculine) AGD was associated with lower (more masculine) digit ratios, as predicted by the literature. Using multi-level linear regression models, we found that AGD and 2D:4D measurements did not differ significantly across cycle phases. AGD-AF and digit ratios in both hands were associated with age at menarche, suggesting a possible common developmental trajectory. These results demonstrate that AGD and 2D:4D are stable across the menstrual cycle. In addition, research is needed to determine how reliably these measures reflect the in utero hormonal milieu.Keywords
This publication has 66 references indexed in Scilit:
- Environmental levels of oestrogenic and antiandrogenic compounds feminize digit ratios in male rats and their unexposed male progenyProceedings. Biological sciences, 2013
- The relationship between anogenital distance and azoospermia in adult menInternational Journal of Andrology, 2012
- Variation in Ano‐Genital Distance in Spontaneously Cycling Female MiceReproduction in Domestic Animals, 2012
- Foetal exposure to phthalate esters and anogenital distance in male newbornsInternational Journal of Andrology, 2011
- Does Finger Fat Produce Sex Differences in Second to Fourth Digit Ratios?Endocrinology, 2009
- Human 2D (index) and 4D (ring) digit lengths: their variation and relationships during the menstrual cycleJournal of Anatomy, 2007
- Decrease in Anogenital Distance among Male Infants with Prenatal Phthalate ExposureEnvironmental Health Perspectives, 2005
- Sex hormones and finger length: What does 2D:4D indicate?Evolution and Human Behavior, 2004
- In-utero androgen exposure and 2nd to 4th digit length ratio--comparisons between healthy controls and females with classical congenital adrenal hyperplasiaHuman Reproduction, 2003
- Generation and characterization of androgen receptor knockout (ARKO) mice: An in vivo model for the study of androgen functions in selective tissuesProceedings of the National Academy of Sciences of the United States of America, 2002