Comparison of Three HPLC Methods for the Analysis of Vitamers of Ascorbic Acid in Shrimp Tissue

Abstract

Several high performance liquid chromatography (HPLC) methods have been developed for the analysis of L-ascorbic acid (CI) and two of its more stable analogs dipotassium ascorbyl-2-sulfate dihydrate (C2), and L-ascorbyl-2-phosphate sodium (C3). Three of these methods were evaluated for the analysis of the three vitamers, and three procedures for the extraction of the vitamers from shrimp (Penaeus Setiferus) tissues were compared.