Modified Enzyme Substrates for the Detection of Bacteria: A Review
Open Access
- 12 August 2020
- Vol. 25 (16), 3690
- https://doi.org/10.3390/molecules25163690
Abstract
The ability to detect, identify and quantify bacteria is crucial in clinical diagnostics, environmental testing, food security settings and in microbiology research. Recently, the threat of multidrug-resistant bacterial pathogens pushed the global scientific community to develop fast, reliable, specific and affordable methods to detect bacterial species. The use of synthetically modified enzyme substrates is a convenient approach to detect bacteria in a specific, economic and rapid manner. The method is based on the use of specific enzyme substrates for a given bacterial marker enzyme, conjugated to a signalogenic moiety. Following enzymatic reaction, the signalophor is released from the synthetic substrate, generating a specific and measurable signal. Several types of signalophors have been described and are defined by the type of signal they generate, such as chromogenic, fluorogenic, luminogenic, electrogenic and redox. Signalophors are further subdivided into groups based on their solubility in water, which is key in defining their application on solid or liquid media for bacterial culturing. This comprehensive review describes synthetic enzyme substrates and their applications for bacterial detection, showing their mechanism of action and their synthetic routes.Keywords
This publication has 100 references indexed in Scilit:
- Selective Pressures to Maintain Attachment Site Specificity of Integrative and Conjugative ElementsPLoS Genetics, 2013
- Synthesis and utility of fluorogenic acetoxymethyl ethersChemical Science, 2010
- Polymeric Colorants: Statistical Copolymers of Indigo Building Blocks with Defined StructuresHelvetica Chimica Acta, 2009
- Enzymatic substrates in microbiologyJournal of Microbiological Methods, 2009
- Electroactive Self-Assembled Monolayers that Permit Orthogonal Control over the Adhesion of Cells to Patterned SubstratesLangmuir, 2006
- Rapid methods for differentiating Gram-positive from Gram-negative aerobic and facultative anaerobic bacteriaJournal of Applied Bacteriology, 1990
- p-aminophenyl phosphate: an improved substrate for electrochemical enzyme immnoassayAnalytica Chimica Acta, 1988
- Immobilized enzyme kinetics analyzed by flow-through microfluorimetry : Resorufin-β-d-galactopyranoside as a New Fluorogenic Substrate for β-GalactosidaseAnalytica Chimica Acta, 1984
- Applicability of the fluorescein diacetate method of detecting active bacteria in freshwaterMicrobial Ecology, 1984
- New Compounds. Substituted Salicylaldehydes and DerivativesJournal of the American Chemical Society, 1950