Eicosapentaenoic Acid Suppresses the Proliferation of Synoviocytes from Rheumatoid Arthritis
Open Access
- 1 January 2008
- journal article
- Published by The Society for Free Radical Research Japan in Journal of Clinical Biochemistry and Nutrition
- Vol. 43 (2), 126-128
- https://doi.org/10.3164/jcbn.2008057
Abstract
Eicosapentaenoic acid (EPA) is essential for normal cell growth, and may play an important role in inflammatory and autoimmune disorders including rheumatoid arthritis. We investigate that EPA could suppress the proliferation of fibroblast like synoviocytes in vitro. We treated synoviocytes with 1 to 50 μM EPA and measured cell viabilities by the modified MTT assay. We sorted the number of them in sub G1 stage by fluorescence-activated cell sorting caliber. And we stained them by light green or Hoechst 33258, and investigate microscopic appearance. The cell viabilities were decreased at 30 μM, 40 μM, and 50 μM of EPA comparing to 0 μM of EPA. The half maximal concentration of synoviocytes inhibition was approximately 25 μM. At day 1 and day 3, cell number was also decreased at 50 μM EPA comparing to control. FACS caliber indicated the number of synoviocytes in sub G1 stage did not increase in each concentration of EPA. Hoechst staining indicated normal chromatin pattern and no change in a nuclear morphology both in EPA treated synoviocytes and in untreated synoviocytes. These findings suggest that EPA could suppress the proliferation of synoviocytes in vivo dose dependently and time dependently, however, the mechanism is not due to apoptosis.Keywords
This publication has 13 references indexed in Scilit:
- Omega-3 Fatty Acids in Inflammation and Autoimmune DiseasesJournal of the American College of Nutrition, 2002
- Dietary n-3 fatty acids and therapy for rheumatoid arthritisSeminars in Arthritis and Rheumatism, 1997
- Validation of a meta-analysis: The effects of fish oil in rheumatoid arthritisJournal of Clinical Epidemiology, 1995
- Effects of dietary enrichment with eicosapentaenoic acid upon autoimmune nephritis in female NZBXNZW/F1 MICEArthritis & Rheumatism, 1983
- Dietary enrichment with the polyunsaturated fatty acid eicosapentaenoic acid prevents proteinuria and prolongs survival in NZB x NZW F1 mice.JCI Insight, 1981
- The effect of dietary supplementation of fish oil on experimental myocardial infarctionProstaglandins, 1980
- Epidemiological Studies in the Upernavik District, GreenlandActa Medica Scandinavica, 1980
- The protective effects of dietary fish oil on focal cerebral infarctionProstaglandins and Medicine, 1979
- HÆMOSTATIC FUNCTION AND PLATELET POLYUNSATURATED FATTY ACIDS IN ESKIMOSThe Lancet, 1979
- CHEMISTRY.The Lancet, 1824