Genetic regulation of alcohol dehydrogenase C2 in the mouse. Developmental consequences of the temporal locus (Adh‐3t) and positioning of Adh‐3 on chromosome 3
- 1 May 1981
- journal article
- research article
- Published by Wiley in Developmental Genetics
- Vol. 2 (1), 89-98
- https://doi.org/10.1002/dvg.1020020108
Abstract
The tissue specificity of a proposed cis‐acting temporal locus (Adh‐3t), which regulates alcohol dehydrogenase C2 (ADH‐C2) activity in mouse reproductive tissue extracts, has been examined in C5 7BL/6J, SM/J, F1 (SM/J × C5 7BL/6J) mice as well as in progeny of an (F1 [SM/J × C5 7BL/6J] × C5 7BL/6J) back‐cross. Electrophoretic variants for ADH‐C2, previously used to localize the gene (Adh‐3) encoding this enzyme on chromosome 3, enabled the relative parental contributions to ADH‐C2 phenotype in F1 and backcross mouse tissues to be determined. These analyses demonstrated that (1) stomach, kidney, lung, adrenals, seminal vesicles, epididymis, uterus, and ovary ADH‐C2 is encoded by a single locus (Adh‐3); Adh‐3t is differentially active in various tissues, eg, lung exhibits no apparent activity whereas the temporal locus is fully active in seminal vesicles; (3) Adh‐3t is probably differentically active in different cells of some tissues, eg, adrenals. Specific activity profiles of stomach and epididymal ADH‐C2 during the neonatal development of C5 7BL/6J, SM/J, and F1 (SM/J × C5 7BL/6J) male mice supported the proposal for a cis‐acting temporal locus for this enzyme. Genetic analyses examining segregation of Adh‐3 and Adh‐3t among backcross progeny suggested that these are distinct but closely linked loci, since one recombinant among 256 progeny was observed. Linkage data of Adh‐3 with Va (varitint‐waddler) and de (droopy ear) was also obtained, which suggested that Adh‐3 is localized on chromosome 3 between Va and de.Keywords
This publication has 20 references indexed in Scilit:
- Genetics of hydroxyacid oxidase isozymes in the mouse: Localisation of Hao-2 on linkage group XVIHeredity, 1978
- Genetic control of enzyme expression in Drosophila: A locus influencing tissue specificity of aldehyde oxidaseJournal of Experimental Zoology, 1978
- Genetic variation, cellular distribution and ontogeny of sorbitol dehydrogenase and alcohol dehydrogenase isozymes in male reproductive tissues of the mouseJournal of Experimental Zoology, 1978
- Radiation-inducedalcohol dehydrogenasemutants in maize following allyl alcohol selection of pollenGenetics Research, 1978
- Genetic determination of the β-galactosidase developmental program in mouse liverCell, 1976
- Genetic control of heat sensitivity and activity level of murine arylsulfatase BBiochemical Genetics, 1976
- A model for genetic analysis of programmed gene expression as reflected in the development of membrane antigensDevelopmental Biology, 1975
- The molecular genetics of mammalian glucuronidaseJournal of Cellular Physiology, 1975
- Studies on the subunit structure and molecular size of the human alcohol dehydrogenase isozymes determined by the different loci, ADH1ADH2, and ADH3Annals of Human Genetics, 1973
- Protein Polymorphism and Genic Heterozygosity in Two European Subspecies of the House MouseEvolution, 1969