Protective effect of erythropoietin against myocardial injury in rats with sepsis and its underlying mechanisms
Open Access
- 8 January 2015
- journal article
- Published by Spandidos Publications in Molecular Medicine Reports
- Vol. 11 (5), 3317-3329
- https://doi.org/10.3892/mmr.2015.3155
Abstract
The aim of this study was to investigate the protective effect of erythropoietin (EPO) against acute myocardial injury and its underlying mechanisms. Mice (n=146) were randomly divided in a double‑blind manner into four groups, sham, Rocephin, EPO and sepsis, and mortality was observed on the seventh day after cecal ligation and puncture. In addition, a total of 252 rats were randomly divided into three groups, sham, EPO and sepsis, and indicators of cardiac function, inflammatory mediators and serum creatine kinase levels were assessed. Mitochondrial membrane potential, cell apoptosis and nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF‑κB) p65 expression levels were detected using flow cytometry. Following intervention with EPO, the mortality rate in mice with sepsis was significantly reduced and the cardiac function of septic rats was significantly improved. In addition, the levels of inflammatory mediators, serum creatine kinase and apoptosis and the myocardial mitochondrial membrane potential and expression of NF‑κB p65 in cardiac tissue were all improved following EPO treatment, and the differences between the results for the sepsis and EPO groups were statistically significant (P<0.05). These findings suggest that EPO reduces the myocardial inflammatory response in septic rats, attenuates the reduction in mitochondrial membrane potential and inhibits myocardial cell apoptosis by reducing NF‑κB p65 expression, and therefore exerts a protective effect in the myocardium.Keywords
This publication has 29 references indexed in Scilit:
- Therapeutic effects of erythropoietin in murine models of endotoxin shock*Critical Care Medicine, 2009
- The non‐haematopoietic biological effects of erythropoietinBritish Journal of Haematology, 2008
- Advances in understanding sepsisEuropean Journal of Anaesthesiology, 2008
- Erythropoietin-receptor agonists in critically ill patients: a meta-analysis of randomized controlled trialsCMAJ : Canadian Medical Association Journal, 2007
- Auto-activation of the Apoptosis Protein Bax Increases Mitochondrial Membrane Permeability and Is Inhibited by Bcl-2Published by Elsevier BV ,2006
- Hearts From Rodents Exposed to Intermittent Hypoxia or Erythropoietin Are Protected Against Ischemia-Reperfusion InjuryCirculation, 2003
- 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions ConferenceIntensive Care Medicine, 2003
- Bax and Bak Coalesce into Novel Mitochondria-Associated Clusters during ApoptosisThe Journal of cell biology, 2001
- Mitochondria and ApoptosisScience, 1998
- Comparative evaluation of several DNA binding dyes in the detection of apoptosis‐associated chromatin degradation by flow cytometryCytometry, 1992