Electronic Transduction of DNA Sensing Processes on Surfaces: Amplification of DNA Detection and Analysis of Single-Base Mismatches by Tagged Liposomes
- 11 May 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 123 (22), 5194-5205
- https://doi.org/10.1021/ja0036256
Abstract
Tagged, negatively charged, liposomes are used to amplify DNA sensing processes. The analyses of the target DNA are transduced electrochemically by using Faradaic impedance spectroscopy, or by microgravimetric measurements with Au-quartz crystals. By one method, a probe oligonucleotide (1) is assembled on Au-electrodes or Au-quartz crystals. The formation of the double-stranded assembly with the analyte DNA (2) is amplified by the association of the 3-oligonucleotide-functionalized liposomes to the sensing interface. The target DNA is analyzed by this method with a sensitivity limit that corresponds to 1 × 10-12 M. A second method to amplify the sensing of the analyte involves the interaction of the 1-functionalized electrode or Au-quartz crystal with the target DNA sample (2) that is pretreated with the biotinylated oligonucleotide (4). The formation of the three-component double-stranded assembly between 1/2/4 is amplified by the association of avidin and biotin-labeled liposomes to the sensing interfaces. By the secondary association of avidin and biotin-tagged liposomes, a dendritic-type amplification of the analysis of the DNA is accomplished. The analyte DNA (2) is sensed by this method with a sensitivity limit corresponding to 1 × 10-13 M. The biotin-tagged liposomes are also used to probe and amplify single-base mismatches in an analyte DNA. The 6-oligonucleotide-functionalized Au-electrode or Au-quartz crystal was used to differentiate the single-base mismatch (G) in the mutant (5) from the normal A-containing gene (5a). Polymerase-induced coupling of the biotinylated-C-base to the double-stranded assembly generated between 6 and 5 followed by the association of avidin and biotin-tagged liposomes is used to probe the single base mismatch. The functionalized liposomes provide a particulate building unit for the dendritic amplification of DNA sensing.Keywords
This publication has 24 references indexed in Scilit:
- Amplified Microgravimetric Quartz-Crystal-Microbalance Assay of DNA Using Oligonucleotide-Functionalized Liposomes or Biotinylated LiposomesJournal of the American Chemical Society, 1999
- Instant DNA DetectionChemical & Engineering News, 1998
- Toward Bioelectronics: Specific DNA Recognition Based on an Oligonucleotide-Functionalized PolypyrroleJournal of the American Chemical Society, 1997
- Padlock probes reveal single-nucleotide differences, parent of origin and in situ distribution of centromeric sequences in human chromosomes 13 and 21Nature Genetics, 1997
- Direct Enzyme-Amplified Electrical Recognition of a 30-Base Model OligonucleotideJournal of the American Chemical Society, 1996
- Electrochecmical biosensors for DNA sequence detectionElectroanalysis, 1996
- Voltammetric DNA Biosensor for Cystic Fibrosis Based on a Modified Carbon Paste ElectrodeAnalytical Chemistry, 1994
- Conformational states of N-acylglycine dithioesters in solution: resonance Raman studies of isotopically substituted models for enzyme-substrate complexesBiochemistry, 1983
- Investigation of electrode reactions by the method of charging-curves and with the aid of alternating currentsDiscussions of the Faraday Society, 1947
- Kinetics of rapid electrode reactionsDiscussions of the Faraday Society, 1947