Report on a project on three-dimensional imaging of the biological cell by single-particle X-ray diffraction
Open Access
- 21 December 2007
- journal article
- Published by International Union of Crystallography (IUCr) in Acta Crystallographica Section A Foundations of Crystallography
- Vol. 64 (1), 33-35
- https://doi.org/10.1107/s010876730705550x
Abstract
Single-particle X-ray diffraction is an extension of X-ray crystallography which allows the specimen to be any small solid-state bounded object; in Shapiroet al.[Proc. Natl Acad. Sci. USA(2005),102, 15343–15346] and Thibaultet al.[Acta Cryst.(2006), A62, 248–261], the reader can find descriptions of a recent StonyBrook/Berkeley/Cornell two-dimensional imaging of a yeast cell by this technique. Our present work is aimed at extending the technique to the three-dimensional imaging of a cell. However, the usual method of doing that, namely rotating the specimen into many orientations in the X-ray beam, has not as yet given sufficiently good three-dimensional diffraction data to allow the work to go forward, the largest problem being the difficulty of preventing unwanted levels of change in the specimen through the extended exposure to a hostile environment of X-rays and, in some cases, high vacuum and/or extreme cold. The present paper discusses possible methods of dealing with this problem.Keywords
This publication has 3 references indexed in Scilit:
- Report on a project on three-dimensional imaging of the biological cell by single-particle X-ray diffraction. AddendumActa Crystallographica Section A Foundations of Crystallography, 2007
- Reconstruction of a yeast cell from X-ray diffraction dataActa Crystallographica Section A Foundations of Crystallography, 2006
- Biological imaging by soft x-ray diffraction microscopyProceedings of the National Academy of Sciences of the United States of America, 2005