Determination of Individual Carotenoids in Human Plasma by High Performance Liquid Chromatography
- 1 March 1985
- journal article
- research article
- Published by Informa UK Limited in Journal of Liquid Chromatography
- Vol. 8 (3), 473-484
- https://doi.org/10.1080/01483918508067094
Abstract
A high performance liquid chromatography procedure using an internal carotenoid standard, echinenone, is described. The method uses a C-18, reverse-phase column, an isocratic solvent (acetonitrile:methylene chloride:methanol, 70:20:10) and requires only 13 min. Most human plasmas gave seven distinct peaks; 6 carotenoids are identified. The sum of 7 peaks is 90% or more of the total carotenoids in a total lipid extract.Keywords
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