AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
Open Access
- 15 August 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Genome Biology
- Vol. 20 (1), 1-9
- https://doi.org/10.1186/s13059-019-1783-3
Abstract
The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is an open-source software that incorporates single-nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing 1 or multiple haplotypes of a sequenced genome, currently supporting 11 Cas proteins. It also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations. AlleleAnalyzer is available at https://github.com/keoughkath/AlleleAnalyzer.Keywords
Funding Information
- National Institutes of Health (UM1HL098179, P01HL089707, R01MH109907, U01HL100406, P01HL089707, R01HL130533, 1R01EY028249)
- Claire Giannini Fund (Claire Giannini Fund)
- UCSF Discovery Fellows Program (UCSF Discovery Fellows Program)
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