A novel role for Gemin5 in mRNA translation
Open Access
- 9 December 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 37 (2), 582-590
- https://doi.org/10.1093/nar/gkn979
Abstract
In eukaryotic cells translation initiation occurs through two alternative mechanisms, a cap-dependent operating in the majority of mRNAs, and a 5′-end-independent driven by internal ribosome entry site (IRES) elements, specific for a subset of mRNAs. IRES elements recruit the translation machinery to an internal position in the mRNA through a mechanism involving the IRES structure and several trans -acting factors. Here, we identified Gemin5 protein bound to the foot-and-mouth disease virus (FMDV) and hepatitis C virus (HCV) IRES using two independent approaches, riboproteomic analysis and immunoprecipitation of photocroslinked factors. Functional analysis performed in Gemin5 shRNA-depleted cells, or in in vitro translation reactions, revealed an unanticipated role of Gemin5 in translation control as a down-regulator of cap-dependent and IRES-driven translation initiation. Consistent with this, pull-down assays showed that Gemin5 forms part of two distinct complexes, a specific IRES-ribonucleoprotein complex and an IRES-independent protein complex containing eIF4E. Thus, beyond its role in snRNPs biogenesis, Gemin5 also functions as a modulator of translation activity.Keywords
This publication has 49 references indexed in Scilit:
- Cytoplasmic Relocalization of Heterogeneous Nuclear Ribonucleoprotein A1 Controls Translation Initiation of Specific mRNAsMolecular Biology of the Cell, 2007
- Influenza Virus mRNA Translation Revisited: Is the eIF4E Cap-Binding Factor Required for Viral mRNA Translation?Journal of Virology, 2007
- Inhibition of Foot-and-Mouth Disease Virus Infections in Cell Cultures with Antisense Morpholino OligomersJournal of Virology, 2007
- Structural insights into the transcriptional and translational roles of Ebp1The EMBO Journal, 2007
- Inhibition of U snRNP assembly by a virus-encoded proteinaseGenes & Development, 2007
- Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin productionGenes & Development, 2007
- A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiationThe EMBO Journal, 2006
- DExD/H box RNA helicases: multifunctional proteins with important roles in transcriptional regulationNucleic Acids Research, 2006
- The Double-Stranded RNA Binding Protein 76:NF45 Heterodimer Inhibits Translation Initiation at the Rhinovirus Type 2 Internal Ribosome Entry SiteJournal of Virology, 2006
- Autoregulation of Polypyrimidine Tract Binding Protein by Alternative Splicing Leading to Nonsense-Mediated DecayMolecular Cell, 2004