Coarse-Grained Dynamics of Protein Synthesis in a Cell-Free System
Open Access
- 24 January 2011
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 106 (4), 048104
- https://doi.org/10.1103/physrevlett.106.048104
Abstract
A complete gene expression reaction is reconstituted in a cell-free system comprising the entire endogenous transcription, translation, as well as mRNA and protein degradation machinery of E. coli. In dissecting the major reaction steps, we derive a coarse-grained enzymatic description of biosynthesis and degradation, from which ten relevant rate constants and concentrations are determined. Governed by zeroth-order degradation, protein expression follows a sharp transition from undetectable levels to constant-rate accumulation, without reaching steady state.Keywords
Funding Information
- National Science Foundation
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