Bacterial community structure at defined locations ofPinus sylvestris-Suillus bovinusandPinus sylvestris-Paxillus involutusmycorrhizospheres in dry pine forest humus and nursery peat
- 1 June 1998
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 44 (6), 499-513
- https://doi.org/10.1139/w98-035
Abstract
Bacteria were isolated and characterized from uncolonized soil, nonmycorrhizal and mycorrhizal short roots, and soil-colonizing external mycelium from intact Pinus sylvestris - Suillus bovinus and Pinus sylvestris - Paxillus involutus mycorrhizospheres developed in microcosms containing dry pine forest humus or nursery peat. Total numbers of colony-forming units (CFU/mg dry weight) in the different locations from all ectomycorrhizospheres indicated an overall bacterial-enrichment gradient towards the roots, whereas sporeformers were more evenly distributed. Fluorescent pseudomonads were commonly isolated from all mycorrhizosphere locations in nursery peat, but they were nearly absent from the forest humus community. In contrast, sporeformers were more abundant at all locations in the latter growth substrate. The bacterial species composition of forest and nursery mycorrhizospheres was clearly divergent when characterized according to their carbon source utilization patterns in Biolog®GN or GP microplates. Factorial-designed ANOVA of a principal component analysis of the carbon source utilization data showed significant differences between isolates from the two soil types and, to a lesser extent, between S. bovinus and Paxillus involutus mycorrhizospheres. Bacterial communities from mycorrhizospheres and uncolonized soil were distinguished by their preferential utilization of carbohydrates and organic and amino acids, respectively. Suillus bovinus associated bacteria appeared to favour mannitol and Paxillus involutus associated bacteria appeared to favour fructose as carbon sources. This study demonstrates the combined effect of soil type, fungal symbiont, and precise location on bacterial communities associated with Pinus sylvestris ectomycorrhizospheres.Key words: Biolog, carbon source utilization, ectomycorrhiza, Scots pine, soil bacteria.Keywords
This publication has 24 references indexed in Scilit:
- Microbial functional activity during composting of chlorophenol-contaminated sawmill soilJournal of Microbiological Methods, 1997
- Use of rhizosphere carbon sources in sole carbon source tests to discriminate soil microbial communitiesJournal of Microbiological Methods, 1997
- Functional biodiversity of microbial communities in the rhizospheres of hybrid larch (Larix eurolepis) and Sitka spruce (Picea sitchensis)Tree Physiology, 1996
- Tansley Review No. 76 Helper bacteria: a new dimension to the mycorrhizal symbiosisNew Phytologist, 1994
- Pseudomonas fluorescens in association with fruit bodies of the ectomycorrhizal mushroom Cantharellus cibariusMycological Research, 1993
- Effect of dual inoculation of Douglas fir with the ectomycorrhizal fungus Laccaria laccata and mycorrhization helper bacteria (MHB) in two bare-root forest nurseriesPlant and Soil, 1991
- Biological interactions in the mycorrhizosphereCellular and Molecular Life Sciences, 1991
- Stimulation of ectomycorrhizal infection of Pinus radiata by some microorganisms associated with the mantle of ectomycorrhizasNew Phytologist, 1989
- Unusual bacteria from fungal materialFEMS Microbiology Letters, 1987
- The theory of forest typesActa Forestalia Fennica, 1926