Exploration of Deinococcus‐Thermus molecular diversity by novel group‐specific PCR primers
Open Access
- 29 August 2013
- journal article
- research article
- Published by Wiley in Microbiologyopen
- Vol. 2 (5), 862-872
- https://doi.org/10.1002/mbo3.119
Abstract
The deeply branching Deinococcus-Thermus lineage is recognized as one of the most extremophilic phylum of bacteria. In previous studies, the presence of Deinococcus-related bacteria in the hot arid Tunisian desert of Tataouine was demonstrated through combined molecular and culture-based approaches. Similarly, Thermus-related bacteria have been detected in Tunisian geothermal springs. The present work was conducted to explore the molecular diversity within the Deinococcus-Thermus phylum in these extreme environments. A set of specific primers was designed in silico on the basis of 16S rRNA gene sequences, validated for the specific detection of reference strains, and used for the polymerase chain reaction (PCR) amplification of metagenomic DNA retrieved from the Tataouine desert sand and Tunisian hot spring water samples. These analyses have revealed the presence of previously undescribed Deinococcus-Thermus bacterial sequences within these extreme environments. The primers designed in this study thus represent a powerful tool for the rapid detection of Deinococcus-Thermus in environmental samples and could also be applicable to clarify the biogeography of the Deinococcus-Thermus phylum.Keywords
This publication has 65 references indexed in Scilit:
- The Diversity and Abundance of Bacteria and Oxygenic Phototrophs in Saline Biological Desert Crusts in Xinjiang, Northwest ChinaMicrobial Ecology, 2013
- Thermus tengchongensis sp. nov., isolated from a geothermally heated soil sample in Tengchong, Yunnan, south-west ChinaAntonie van Leeuwenhoek, 2012
- Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern RussiaApplied and Environmental Microbiology, 2012
- A robust PCR primer design platform applied to the detection of Acidobacteria Group 1 in soilNucleic Acids Research, 2012
- Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge HostsApplied and Environmental Microbiology, 2011
- Sunlight-Exposed Biofilm Microbial Communities Are Naturally Resistant to Chernobyl Ionizing-Radiation LevelsPLOS ONE, 2011
- Oxidative Stress Resistance inDeinococcus radioduransMicrobiology and Molecular Biology Reviews, 2011
- Non-Random Assembly of Bacterioplankton Communities in the Subtropical North Pacific OceanFrontiers in Microbiology, 2011
- Cyanobacteria and chloroflexi-dominated hypolithic colonization of quartz at the hyper-arid core of the Atacama Desert, ChileExtremophiles, 2010
- Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approachesExtremophiles, 2010