The aerial mycelium-defective phenotype of Streptomyces griseus resulting from A-factor deficiency is suppressed by a Ser/Thr kinase of S. coelicolor A3(2)
- 22 February 1996
- journal article
- research article
- Published by Elsevier BV in Gene
- Vol. 169 (1), 91-95
- https://doi.org/10.1016/0378-1119(95)00771-7
Abstract
A-factor (2-isocapryloyl-3R-hydroxymethyl-γ-butyrolactone) is essential for aerial mycelium formation and streptomycin (Sm) production in Streptomyces griseus. A protein Ser/Thr kinase (AfsK), the product of the Streptomyces coelicolor A3(2) afsK gene, controlling secondary metabolism in this strain, reversed the aerial mycelium-negative phenotype of an A-factor-deficient mutant strain, S. griseus HH1, and induced sporulation without affecting A-factor productivity or Sm production. A mutant AfsK protein lacking kinase activity failed to induce aerial mycelium formation which indicates the importance of the kinase activity for suppression in S. griseus. These data suggest that a Ser/Thr kinase functionally similar to S. coelicolor A3(2) AfsK plays a regulatory role in aerial mycelium formation in S. griseus, either as a member in the A-factor regulatory network or independently of this networkKeywords
This publication has 10 references indexed in Scilit:
- Involvement of a Small ORF Downstream of the afsR Gene in the Regulation of Secondary Metabolism in Streptomyces coelicolor A3(2).Actinomycetologica, 1995
- Phosphorylation of the AfsR protein involved in secondary metabolism in Streptomyces species by a eukary otic-type protein kinaseGene, 1994
- A‐factor as a microbial hormone that controls cellular differentiation and secondary metabolism in Streptomyces griseusMolecular Microbiology, 1994
- A gene cluster involved in aerial mycelium formation in Streptomyces griseus encodes proteins similar to the response regulators of two-component regulatory systems and membrane translocatorsJournal of Bacteriology, 1993
- AUTOREGULATORY FACTORS AND COMMUNICATION IN ACTINOMYCETESAnnual Review of Microbiology, 1992
- Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2)Gene, 1990
- afsB stimulates transcription of the actinorhodin biosynthetic pathway in Streptomyces coelicolor A3(2) and Streptomyces lividansMolecular Genetics and Genomics, 1989
- K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinasesBiochemical and Biophysical Research Communications, 1987
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Gene expression in Streptomyces: Construction and application of promoter-probe plasmid vectors in Streptomyces lividansMolecular Genetics and Genomics, 1982