Separation of Enantiomers of β‐Lactams by HPLC Using Cyclodextrin‐Based Chiral Stationary Phases
- 1 June 2006
- journal article
- research article
- Published by Informa UK Limited in Journal of Liquid Chromatography & Related Technologies
- Vol. 29 (13), 1847-1860
- https://doi.org/10.1080/10826070600757540
Abstract
The enantiomeric separation of 12 β‐lactam compounds on 3 native cyclodextrin and 6 derivatized β‐cyclodextrin stationary phases was evaluated using high performance liquid chromatography (HPLC). The dimethylphenyl carbamate functionalized chiral stationary phase (CSP) (Cyclobond I 2000 DMP) separated 11 of the 12 β‐lactams in the reversed phase mode. The dimethylated β‐cyclodextrin column (Cyclobond I 2000 DM) was the second most effective CSP and it separated 8 of the 12 compounds. The reversed phase separation mode was the most effective approach. The effects of the composition and the flow rate on enantioseparations were studied. The effect of the structure of the substituents on the β‐lactams was examined.Keywords
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