Protective Effects of Sasanquasaponin on Injury of Endothelial Cells Induced by Anoxia and Reoxygenation in vitro
- 29 August 2007
- journal article
- Published by Wiley in Basic & Clinical Pharmacology & Toxicology
- Vol. 101 (5), 301-308
- https://doi.org/10.1111/j.1742-7843.2007.00119.x
Abstract
The protective effects of sasanquasaponin, an effective compound from Chinese traditional herbs, on ischaemia and reperfusion injury in mouse hearts have been suggested through modulation of intracellular Cl(-) homeostasis. The effects of sasanquasaponin on injury of endothelial cells, however, induced by anoxia and reoxygenation remain unknown. Therefore, the present study attempted to observe the effects of sasanquasaponin on anoxia and reoxygenation injury in endothelial cells and investigate its putative mechanisms. Human umbilical vein endothelial cells (HUVECs) were exposed to normoxia or anoxia and reoxygenation in the absence or presence of sasanquasaponin (10.0, 1.0 and 0.1 micromol/l). Lactate dehydrogenase activity was determined in cultured HUVECs supernatant, and malondialdehyde content, superoxide dismutase and glutathione peroxidase activities were measured in HUVECs by a colorimetric method. Neutrophil adhesion to HUVECs was assayed colorimetrically. The levels of intercellular adhesion molecule-1 and tumour necrosis factor-alpha were detected. The activity of nuclear factor kappa B was determined by flow cytometry. The results show that sasanquasaponin decreased the lactate dehydrogenase activity and malondialdehyde contents, and inhibited the neutrophil adhesion to HUVECs; sasanquasaponin, moreover, inhibited nuclear factor kappa B transnuclear activity, lowered tumour necrosis factor-alpha and intercellular adhesion molecule-1 expression levels. On the other hand, sasanquasaponin increased the mitochondrial superoxide dismutase and glutathione peroxidase activities. It is suggested that sasanquasaponin could protect HUVECs against anoxia and reoxygenation injury, and the protective mechanisms appear to be related to anti-lipoperoxidation and anti-adhesion.Keywords
This publication has 24 references indexed in Scilit:
- Attenuation of brain inflammatory response after focal cerebral ischemia/reperfusion with Xuesaitong Injection in ratsChinese Journal of Integrated Medicine, 2006
- A classified approach to burn contracturesBurns, 2005
- Inhibitory effects of sasanquasaponin on over-expression of ICAM-1 and on enhancement of capillary permeability induced by burns in ratsBurns, 2005
- Stimulation of adhesion molecule expression in human endothelial cells (HUVEC) by adrenomedullin and corticotrophinAmerican Journal of Physiology-Cell Physiology, 2004
- Protective effect of susanquasaponin on experimental myocardial ischemia in mouse in vivo and its possible mechanismsJournal of Molecular and Cellular Cardiology, 2002
- Xanthine Oxidase–Derived Reactive Oxygen Species Convert Flow-Induced Arteriolar Dilation to Constriction in HyperhomocysteinemiaArteriosclerosis, Thrombosis, and Vascular Biology, 2002
- Neutrophil–endothelial cell interactions during the development of tolerance to ischaemia/reperfusion in isolated cellsActa Physiologica Scandinavica, 2001
- Migration of Leukocytes across Endothelial Junctions: Some Concepts and ControversiesMicrocirculation, 2001
- Migration of Leukocytes across Endothelial Junctions: Some Concepts and ControversiesMicrocirculation, 2001
- Intercellular Adhesion Molecule-1 (ICAM-1) Gene Expression in Human T Cells Is Regulated by Phosphotyrosyl Phosphatase ActivityPublished by Elsevier BV ,2001