Epithelial-mesenchymal transition in primary human bronchial epithelial cells is Smad-dependent and enhanced by fibronectin and TNF-α
Open Access
- 5 January 2010
- journal article
- Published by Springer Science and Business Media LLC in Fibrogenesis & Tissue Repair
- Vol. 3 (1), 2
- https://doi.org/10.1186/1755-1536-3-2
Abstract
Defective epithelial repair, excess fibroblasts and myofibroblasts, collagen overproduction and fibrosis occur in a number of respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. Pathological conversion of epithelial cells into fibroblasts (epithelial-mesenchymal transition, EMT) has been proposed as a mechanism for the increased fibroblast numbers and has been demonstrated to occur in lung alveolar epithelial cells. Whether other airway cell types also have the capability to undergo EMT has been less explored so far. A better understanding of the full extent of EMT in airways, and the underlying mechanisms, can provide important insights into airway disease pathology and enable the development of new therapies. The main aim of this study was to test whether primary human bronchial epithelial cells are able to undergo EMT in vitro and to investigate the effect of various profibrotic factors in the process.Keywords
This publication has 60 references indexed in Scilit:
- Pulmonary fibrosis: pathogenesis, etiology and regulationMucosal Immunology, 2009
- BMP-7 Does Not Protect against Bleomycin-Induced Lung or Skin FibrosisPLOS ONE, 2008
- Downregulation of MMP-2 and -9 by Proteasome Inhibition: A Possible Mechanism to Decrease LEC Migration and Prevent Posterior Capsular OpacificationInvestigative Ophthalmology & Visual Science, 2008
- Collagen I–mediated up-regulation of N-cadherin requires cooperative signals from integrins and discoidin domain receptor 1The Journal of cell biology, 2008
- Idiopathic Pulmonary Fibrosis: Aberrant Recapitulation of Developmental Programs?PLoS Medicine, 2008
- Gremlin-mediated Decrease in Bone Morphogenetic Protein Signaling Promotes Pulmonary FibrosisAmerican Journal of Respiratory and Critical Care Medicine, 2008
- Expression and mutation analysis of the discoidin domain receptors 1 and 2 in non-small cell lung carcinomaBritish Journal of Cancer, 2007
- Alveolar epithelial cell mesenchymal transition develops in vivo during pulmonary fibrosis and is regulated by the extracellular matrixProceedings of the National Academy of Sciences of the United States of America, 2006
- The mesenchymal cell, its role in the embryo, and the remarkable signaling mechanisms that create itDevelopmental Dynamics, 2005
- Smad-dependent and Smad-independent pathways in TGF-β family signallingNature, 2003