Acoustic imaging of thick biological tissue
- 26 June 2009
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
- Vol. 56 (7), 1352-1358
- https://doi.org/10.1109/tuffc.2009.1191
Abstract
Up to now, biomedical imaging with ultrasound for observing a cellular tissue structure has been limited to very thinly sliced tissue at very high ultrasonic frequencies, i.e., 1 GHz. In this paper, we present the results of a systematic study to use a 150 to 200 MHz frequency range for thickly sliced biological tissue. A mechanical scanning reflection acoustic microscope (SAM) was used for obtaining horizontal cross-sectional images (C-scans) showing cellular structures. In the study, sectioned specimens of human breast cancer and tissues from the small intestine were prepared and examined. Some accessories for biomedical application were integrated into our SAM (Sonix HS-1000 and Olympus UH-3), which operated in pulse-wave and tone-burst wave modes, respectively. We found that the frequency 100 to 200 MHz provides optimal balance between resolution and penetration depth for examining the thickly sliced specimens. The images obtained with the lens focused at different depths revealed cellular structures whose morphology was very similar to that seen in the thinly sectioned specimens with optical and scanning acoustic microscopy. The SAM operation in the pulse-echo mode permits the imaging of tissue structure at the surface, and it also opens up the potential for attenuation imaging representing reflection from the substrate behind the thick specimen. We present such images of breast cancer proving the method's applicability to overall tumor detection. SAM with a high-frequency tone-burst ultrasonic wave reveals details of tissue structure, and both methods may serve as additional diagnostic tools in a hospital environment.Keywords
This publication has 24 references indexed in Scilit:
- The use of pulse-echo acoustic microscopy to non-invasively determine the sex of living larval sea lampreysJournal of Fish Biology, 2004
- Breast tissue characterization with high-frequency scanning acoustic microscopyThe Journal of the Acoustical Society of America, 2004
- Advances in ultrasound biomicroscopyJapanese Journal of Clinical Oncology, 2000
- High-frequency ultrasound: Determination of the lowest frequency required for cellular imaging and detection of myocardial diseaseAmerican Heart Journal, 1995
- The Role of Elasticity in the Motile Behaviour of CellsPublished by Springer Science and Business Media LLC ,1994
- Calculating acoustical properties of cells: Influence of surface topography and liquid layer between cell and substrateThe Journal of the Acoustical Society of America, 1992
- What can Scanning Acoustic Microscopy Tell About Animal Cells and Tissues?Published by Springer Science and Business Media LLC ,1989
- The elastic microstructure of various tissuesThe Journal of the Acoustical Society of America, 1989
- Frequency dependence of ultrasound attenuation and backscatter in breast tissueJapanese Journal of Clinical Oncology, 1986
- Acoustic microscope—scanning versionApplied Physics Letters, 1974