The Hypersensitive Response to Tomato leaf curl New Delhi virus Nuclear Shuttle Protein Is Inhibited by Transcriptional Activator Protein
Open Access
- 1 December 2007
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 20 (12), 1581-1588
- https://doi.org/10.1094/mpmi-20-12-1581
Abstract
The hypersensitive response (HR) is a common feature of plant disease resistance reactions and a type of programmed cell death (PCD). Many pathogens are able to modulate pathways involved in cell death. In contrast to animal viruses, inhibitors of PCD activity have not been identified for plant-infecting viruses. Previously, we have reported that the nuclear shuttle protein (NSP) of Tomato leaf curl New Delhi virus (ToLCNDV) induces an HR in Nicotiana tabacum and Lycopersicon esculentum plants when expressed under the control of the Cauliflower mosaic virus 35S promoter. However, HR is not evident in plants infected with ToLCNDV, suggesting that the virus encodes a factor (or factors) that counters this response. Analysis of all ToLCNDV-encoded genes pinpointed the transcriptional activator protein (TrAP) as the factor mediating the anti-HR effect. Deletion mutagenesis showed the central region of TrAP, containing a zinc finger domain and nuclear localization signal, to be important in inhibiting the HR. These results demonstrate that TrAP counters HR-induced cell death, the first such activity identified for a plant-infecting virus.Keywords
This publication has 50 references indexed in Scilit:
- Subviral agents associated with plant single-stranded DNA virusesVirology, 2006
- MicroRNA-binding viral protein interferes with Arabidopsis developmentProceedings of the National Academy of Sciences, 2005
- Functional Characterization of the Nuclear Localization Signal for a Suppressor of Posttranscriptional Gene SilencingJournal of Virology, 2003
- The Herpes Simplex Virus 1 U S 3 Protein Kinase Blocks Caspase-Dependent Double Cleavage and Activation of the Proapoptotic Protein BADJournal of Virology, 2003
- HLM1, an Essential Signaling Component in the Hypersensitive Response, Is a Member of the Cyclic Nucleotide–Gated Channel Ion Channel Family[W]Plant Cell, 2003
- Bean dwarf mosaic virus BV1 Protein Is a Determinant of the Hypersensitive Response and Avirulence in Phaseolus vulgarisMolecular Plant-Microbe Interactions®, 2000
- TheArabidopsis dnd1“defense, no death” gene encodes a mutated cyclic nucleotide-gated ion channelProceedings of the National Academy of Sciences, 2000
- The Rx Gene from Potato Controls Separate Virus Resistance and Cell Death ResponsesPlant Cell, 1999
- Signaling in Plant-Microbe InteractionsScience, 1997
- Death Don't Have No Mercy: Cell Death Programs in Plant-Microbe Interactions.Plant Cell, 1996