Hemoglobin Carbamylation in Uremia

Abstract

CHROMATOGRAPHY of hemolysates of red cells resolves four minor hemoglobin components from the main hemoglobin A (HbA) fraction. These minor components — HbA_1a1, HbA_1a2, HbA_lb, and HbA_1c — collectively referred to as the HbA₁ fraction, make up approximately 7 per cent of total hemoglobin in normal subjects and result mainly from the post-translational, nonenzymatic glycosylation of HbA.¹ In HbA_lc, the most abundant of the glycosylated hemoglobins, glucose is attached to the N-terminal amino group of the β chains by a stable ketoamine linkage; HbA_1c is present in increased amounts . . .