Functional analysis of AeSCP‐2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti
- 31 May 2005
- journal article
- research article
- Published by Wiley in Insect Molecular Biology
- Vol. 14 (3), 301-307
- https://doi.org/10.1111/j.1365-2583.2005.00560.x
Abstract
The effect of gene expression knockdown was used to study the function of the sterol carrier protein-2 (AeSCP-2) in the yellow fever mosquito, Aedes aegypti. Injection of small double stranded AeSCP-2 RNAs into mosquito larvae resulted in the knockdown of gene products. The lack of AeSCP-2 in larvae coincided with a reduction in accumulated cholesterol in pupae, supporting the hypothesis that AeSCP-2 may be involved in cholesterol uptake in mosquito larvae. Knockdown of AeSCP-2 caused a high mortality rate in developing adult and reduced egg viability. Results from this study indicate that AeSCP-2 is important for adult development and for the viability of the eggs.Keywords
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