Separation of Chlorinated Phenols by Isocratic High-Performance Liquid Chromatography on Reverse Phase Column

Abstract
A method is described for isocratic highperformance liquid chromatographic separation of chlorinated phenols using methanol : phosphate buffer - pH 7.20 (50:50) solvent system. 13 out of 15 congeners of chlorophenols and phenol have been separated in 32 minutes at a flow rate of 0.8 ml per minute. The system is found to be useful for the separation of chlorophenols extracted from mouse liver fed with hexachlorocyclohexane.