Insights into Stabilization of a Viral Protein Cage in Templating Complex Nanoarchitectures: Roles of Disulfide Bonds
- 9 September 2013
- Vol. 10 (3), 536-543
- https://doi.org/10.1002/smll.201300860
Abstract
As a typical protein nanostructure, virus‐based nanoparticle (VNP) of simian virus 40 (SV40), which is composed of pentamers of the major capsid protein of SV40 (VP1), has been successfully employed in guiding the assembly of different nanoparticles (NPs) into predesigned nanostructures with considerable stability. However, the stabilization mechanism of SV40 VNP remains unclear. Here, the importance of inter‐pentamer disulfide bonds between cysteines in the stabilization of quantum dot (QD)‐containing VNPs (VNP‐QDs) is comprehensively investigated by constructing a series of VP1 mutants of cysteine to serine. Although the presence of a QD core can greatly enhance the assembly and stability of SV40 VNPs, disulfide bonds are vital to stability of VNP‐QDs. Cysteine at position 9 (C9) and C104 contribute most of the disulfide bonds and play essential roles in determining the stability of SV40 VNPs as templates to guide assembly of complex nanoarchitectures. These results provide insightful clues to understanding the robustness of SV40 VNPs in organizing suprastructures of inorganic NPs. It is expected that these findings will help guide the future design and construction of protein‐based functional nanostructures.Keywords
This publication has 32 references indexed in Scilit:
- Pathway switching in templated virus-like particle assemblySoft Matter, 2012
- Disassembly of Simian Virus 40 during Passage through the Endoplasmic Reticulum and in the CytoplasmJournal of Virology, 2012
- Protein delivery using engineered virus-like particlesProceedings of the National Academy of Sciences of the United States of America, 2011
- Generation of protein lattices by fusing proteins with matching rotational symmetryNature Nanotechnology, 2011
- Three‐Dimensional Directed Self‐Assembly of Peptide Nanowires into Micrometer‐Sized Crystalline Cubes with Nanoparticle JointsAngewandte Chemie-International Edition, 2010
- Viral Capsid DNA Aptamer Conjugates as Multivalent Cell-Targeting VehiclesJournal of the American Chemical Society, 2009
- Imaging Viral Behavior in Mammalian Cells with Self‐Assembled Capsid–Quantum‐Dot Hybrid ParticlesSmall, 2009
- Structural basis of GM1 ganglioside recognition by simian virus 40Proceedings of the National Academy of Sciences of the United States of America, 2008
- Amino Acid Pairing Preferences in Parallel β-Sheets in ProteinsJournal of Molecular Biology, 2006
- Pairs of Vp1 Cysteine Residues Essential for Simian Virus 40 InfectionJournal of Virology, 2005