Role of the Nha1 antiporter in regulating K+influx inSaccharomyces cerevisiae
- 19 December 2001
- Vol. 19 (1), 9-15
- https://doi.org/10.1002/yea.799
Abstract
NHA1 encodes a K(+) (Na(+))/H(+) antiporter in the plasma membrane of Saccharomyces cerevisiae. We report that cells expressing the NHA1 gene contained less K(+) than the mutant lacking the gene when grown without K(+) limitation. They also grew better at low K(+) and showed higher affinity of transport than the nha1 strain. In agreement with the function of an electroneutral cation/H(+) antiporter, the effect was only observed at acidic pH. The improved growth and transport depended on the presence of Trk1p (the main K(+) influx system) and did not require the product of TRK2. We propose that Nha1p regulates the potassium content of the cell and, as a consequence, can affect the activity of the main K(+) influx system (Trk1p).Keywords
Funding Information
- European Community (BIO4-CT950161, BIO4-CT972210)
- Dirección General de Investigación Científica y Técnica, Spain
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