Functional Myc-Max heterodimer is required for activation-induced apoptosis in T cell hybridomas.
Open Access
- 1 December 1994
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 180 (6), 2413-2418
- https://doi.org/10.1084/jem.180.6.2413
Abstract
T cell hybridomas respond to activation signals by undergoing apoptotic cell death, and this is likely to represent comparable events related to tolerance induction in immature and mature T cells in vivo. Previous studies using antisense oligonucleotides implicated the c-Myc protein in the phenomenon of activation-induced apoptosis. This role for c-Myc in apoptosis is now confirmed in studies using a dominant negative form of its heterodimeric binding partner, Max, which we show here inhibits activation-induced apoptosis. Further, coexpression of a reciprocally mutant Myc protein capable of forming functional heterodimers with the mutant Max can compensate for the dominant negative activity and restore activation-induced apoptosis. These results imply that Myc promotes activation-induced apoptosis by obligatory heterodimerization with Max, and therefore, by regulating gene transcription.Keywords
This publication has 34 references indexed in Scilit:
- Lymphoma models for B cell activation and tolerance. X. Anti-mu-mediated growth arrest and apoptosis of murine B cell lymphomas is prevented by the stabilization of myc.The Journal of Experimental Medicine, 1994
- The S-phase cytotoxicity of camptothecinExperimental Cell Research, 1991
- Max: A Helix-Loop-Helix Zipper Protein That Forms a Sequence-Specific DNA-Binding Complex with MycScience, 1991
- Activation-induced cell death in T cell hybridomas is due to apoptosis. Morphologic aspects and DNA fragmentation.The Journal of Immunology, 1990
- T cell receptor-mediated DNA fragmentation and cell death in T cell hybridomas.The Journal of Immunology, 1990
- Activation-driven T cell death. I. Requirements for de novo transcription and translation and association with genome fragmentation.The Journal of Immunology, 1989
- Rodent fibroblast tumours expressing human myc and ras genes: growth, metastasis and endogenous oncogene expressionBritish Journal of Cancer, 1987
- Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences of the United States of America, 1987
- Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death.The Journal of Immunology, 1984
- T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for ActivityThe Journal of Immunology, 1978