Lipopolysaccharide alters decorin and biglycan synthesis in rat alveolar bone osteoblasts: consequences for bone repair during periodontal disease
Open Access
- 7 May 2008
- journal article
- Published by Wiley in European Journal of Oral Sciences
- Vol. 116 (3), 207-216
- https://doi.org/10.1111/j.1600-0722.2008.00535.x
Abstract
A prime pathogenic agent associated with periodontitis is lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. This study investigated the effects of P. gingivalis LPS on osteoblasts, which are responsible for alveolar bone repair. Bone cells were obtained from explants of rat alveolar bone chips and cultured with 0–200 ng ml−1 of P. gingivalis LPS. Porphyromonas gingivalis LPS significantly increased cell proliferation and inhibited osteoblast differentiation, as judged by reduced alkaline phosphatase activity. Analysis of biglycan mRNA and protein levels indicated that P. gingivalis LPS significantly delayed the normally high expression of biglycan during the early stages of culture, which are associated with cell proliferation and early differentiation of progenitor cells. In the presence of P. gingivalis LPS, decorin expression by the alveolar bone cells was reduced during periods of culture relating to collagen fibrillogenesis and mineral deposition. Analysis of glycosaminoglycan chains conjugated to these proteoglycans suggested that in the presence of P. gingivalis LPS, dermatan sulfate persisted within the matrix. This study suggests that P. gingivalis LPS influences the expression and processing of decorin and biglycan in the matrix, altering alveolar bone cell activity and osteoblast phenotype development. The consequences of this altered expression in relation to hindering bone repair as part of the cycle of events during periodontal disease are discussed.Keywords
This publication has 59 references indexed in Scilit:
- Lipopolysaccharide from Prevotella nigrescens stimulates osteoclastogenesis in cocultures of bone marrow mononuclear cells and primary osteoblastsJournal of Periodontal Research, 2006
- Effects of growth factors and cytokines on osteoblast differentiationPeriodontology 2000, 2006
- Lipopolysaccharide stimulates the production of prostaglandin E2 and the receptor Ep4 in osteoblastsLife Sciences, 2006
- Molecular and cell biology of healthy and diseased periodontal tissuesPeriodontology 2000, 2006
- Extracellular Matrix Proteoglycans Control the Fate of Bone Marrow Stromal CellsOnline Journal of Public Health Informatics, 2005
- The efficacy of mesenchymal stem cells to regenerate and repair dental structuresOrthodontics & Craniofacial Research, 2005
- Substance P Enhances the Inhibition of Osteoblastic Cell Differentiation Induced by Lipopolysaccharide From Porphyromonas gingivalisThe Journal of Periodontology, 2004
- The small leucine‐rich proteoglycan biglycan modulates BMP‐4‐induced osteoblast differentiationThe FASEB Journal, 2004
- Glycosaminoglycans in peri‐implant sulcus fluid from implants placed in sinus‐inlay bone graftsClinical Oral Implants Research, 2001
- The Family of the Small Leucine-Rich Proteoglycans: Key Regulators of Matrix Assembly and Cellular GrowthCritical Reviews in Biochemistry and Molecular Biology, 1997