Changes in total and quantal release of acetylcholine in the mouse diaphragm during activation and inhibition of membrane ATPase.
- 1 January 1979
- journal article
- research article
- Published by Wiley in The Journal of Physiology
- Vol. 286 (1), 1-14
- https://doi.org/10.1113/jphysiol.1979.sp012603
Abstract
Acetylcholine (ACh) released from mouse diaphragm was gel filtrated and estimated by bio-assay and compared with electrophysiologically measured quantal release, expressed either as frequency of miniature end-plate potential or quantum content of end-plate potentials. Activation of Na+-K+-dependent membrane ATPase (membrane ATPase) in Na+-loaded muscles lowered the total amount of ACh released at rest to 1/10 of the control value, but quantal release remained unchanged. Inhibition of membrane ATPase by 2 .times. 10-5 M-ouabain or by K-free solution led to an increase in total release and to a delayed progressive increase in quantal release. When Ca2+ was removed only the total release was enhanced. Depolarization of the diaphragm by 8, 11 and 14 mM-K increased both total and quantal release only in the presence of Ca2+ in the perfusion medium. When Ca2+ was removed, no significant increase in release was observed. The total and quantal release in response to 2 Hz stimulation of the preparation was increased 1.4 and 45 .times., respectively. The total amount of ACh released at rest apparently consists of 2 fractions, quantal and non-quantal, the former representing about 1% of the total release.This publication has 25 references indexed in Scilit:
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