Analytic Method on Characteristic Parameters of Bacteria in Water by Multiwavelength Transmission Spectroscopy
Open Access
- 1 January 2017
- journal article
- Published by Hindawi Limited in Journal of Spectroscopy
- Vol. 2017, 1-7
- https://doi.org/10.1155/2017/4039048
Abstract
An analytic method together with the Mie scattering theory and Beer-Lambert law is proposed for the characteristic parameter determination of bacterial cells (Escherichia coli10389) from multiwavelength transmission spectroscopy measurements. We calculate the structural parameters ofE. colicells, and compared with the microscopy, the relative error of cell volume is 7.90%, the cell number is compared with those obtained by plate counting, the relative error is l.02%, and the nucleic content and protein content of singleE. colicells are consistent with the data reported elsewhere. The proposed method can obtain characteristic parameters of bacteria as an excellent candidate for the rapid detection and identification of bacteria in the water.Keywords
Funding Information
- National Natural Science Foundation of China (61705237, 61378041, 15CZZ04125, 1608085QF137, 1508085JGD02)
This publication has 32 references indexed in Scilit:
- Detection of influenza A viruses with a portable real-time PCR instrumentJournal of Virological Methods, 2012
- Simple quantitative analysis of Escherichia coli K-12 internalized in baby spinach using Fourier Transform Infrared spectroscopyInternational Journal of Food Microbiology, 2010
- Fluorescence Spectroscopy for Rapid Detection and Classification of Bacterial PathogensApplied Spectroscopy, 2009
- Survival and viability of Helicobacter pylori after inoculation into chlorinated drinking waterWater Research, 2007
- Quantitative spectroscopy analysis of prokaryotic cells: vegetative cells and sporesBiosensors and Bioelectronics, 2004
- Bacteria size determination by elastic light scatteringIEEE Journal of Selected Topics in Quantum Electronics, 2003
- Detection of Karenia mikimotoi by spectral absorption signaturesJournal of Plankton Research, 2003
- Light scattering from cells: the contribution of the nucleus and the effects of proliferative statusJournal of Biomedical Optics, 2000
- Macromolecular composition of bacteriaJournal of Theoretical Biology, 1982
- Detection of Uropathogens by Using Chromogenic Media (Hicrome UTI agar), CLED agar and other Conventional MediaFaridpur Medical College Journal, 1970