Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells
- 30 January 2006
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 103 (6), 1762-1767
- https://doi.org/10.1073/pnas.0504777103
Abstract
We show that actin filaments, shortened to physiological lengths by gelsolin and cross-linked with recombinant human filamins (FLNs), exhibit dynamic elastic properties similar to those reported for live cells. To achieve elasticity values of comparable magnitude to those of cells, the in vitro network must be subjected to external prestress, which directly controls network elasticity. A molecular requirement for the strain-related behavior at physiological conditions is a flexible hinge found in FLNa and some FLNb molecules. Basic physical properties of the in vitro filamin-F-actin network replicate the essential mechanical properties of living cells. This physical behavior could accommodate passive deformation and internal organelle trafficking at low strains yet resist externally or internally generated high shear forces.Keywords
This publication has 40 references indexed in Scilit:
- Chicken gizzard filamin, retina filamin and cgABP260 are respectively, smooth muscle‐, non‐muscle‐ and pan‐muscle‐type isoforms: Distribution and localization in musclesCell Motility, 2005
- Rheology of airway smooth muscle cells is associated with cytoskeletal contractile stressJournal of Applied Physiology, 2004
- Comparison of Filamin A-induced Cross-linking and Arp2/3 Complex-mediated Branching on the Mechanics of Actin FilamentsPublished by Elsevier BV ,2002
- Scaling the Microrheology of Living CellsPhysical Review Letters, 2001
- Investigating the Cytoskeleton of Chicken Cardiocytes with the Atomic Force MicroscopeJournal of Structural Biology, 1997
- FORCE EFFECTS ON BIOCHEMICAL KINETICSAnnual Review of Biochemistry, 1997
- Mechanically stimulated cytoskeleton rearrangement and cortical contraction in human neutrophilsBiophysical Journal, 1995
- Measurement of the persistence length of polymerized actin using fluorescence microscopyPhysical Review E, 1993
- Identification of critical functional and regulatory domains in gelsolin.The Journal of cell biology, 1989
- The architecture of actin filaments and the ultrastructural location of actin-binding protein in the periphery of lung macrophages.The Journal of cell biology, 1986