Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS, immunological and microbiological techniques
- 2 December 2009
- journal article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 32 (4), 503-506
- https://doi.org/10.1007/s10529-009-0176-1
Abstract
Vitamin B12 was produced by probiotic Lactobacillus plantarum in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h each to give 13 ng vitamin B12/g dry biomass. Sodium cyanide-mediated cell lysis, followed by benzyl alcohol/chloroform/water extraction, improved the release of intracellular vitamin B12 for analysis. The presence of the K+ adduct of cyanocobalamin (m/z of 1394) was established using electron spray ionization–mass spectra; growth of a mutant of Escherichia coli in the presence of cyanocobalamin ascertained its bioavailability.Keywords
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