Regulation of Mouse Follicle Development by Follicle-Stimulating Hormone in a Three-Dimensional In Vitro Culture System Is Dependent on Follicle Stage and Dose1
Open Access
- 1 November 2005
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 73 (5), 942-950
- https://doi.org/10.1095/biolreprod.105.042390
Abstract
The developmental requirements of ovarian follicles are dependent on the maturation stage of the follicle; in particular, elegant studies with genetic models have indicated that FSH is required for antral, but not preantral, follicle growth and maturation. To elucidate further the role of FSH and other regulatory molecules in preantral follicle development, in vitro culture systems are needed. We employed a biomaterials-based approach to follicle culture, in which follicles were encapsulated within matrices that were tailored to the specific developmental needs of the follicle. This three-dimensional system was used to examine the impact of increasing doses of FSH on follicle development for two-layered secondary (100–130 μm; two layers of granulosa cells surrounding the oocyte) and multilayered secondary (150–180 μm, several layers of granulosa cells surrounding the oocyte) follicles isolated from mice. Two-layered secondary follicles were FSH responsive when cultured in alginate-collagen I matrices, exhibiting FSH dose-dependent increases in follicle growth, lactate production, and steroid secretion. Multilayered secondary follicles were FSH dependent, with follicle survival, growth, steroid secretion, metabolism, and oocyte maturation all regulated by FSH. However, doses greater than 25 mIU/ml of FSH negatively impacted multilayered secondary follicle development (reduced follicle survival). The present results indicate that the hormonal and environmental needs of the follicular complex change during the maturation process. The culture system can be adapted to each stage of development, which will be especially critical for translation to human follicles that have a longer developmental period.Keywords
This publication has 58 references indexed in Scilit:
- Follicle-Stimulating Hormone Affects Metaphase I Chromosome Alignment and Increases Aneuploidy in Mouse Oocytes Matured in Vitro1Biology of Reproduction, 2005
- Novel Approach for the Three-Dimensional Culture of Granulosa Cell–Oocyte ComplexesTissue Engineering, 2003
- Murine granulosa cell morphology and function are regulated by a synthetic Arg–Gly–Asp matrixMolecular and Cellular Endocrinology, 2003
- A Revised Protocol for In Vitro Development of Mouse Oocytes from Primordial Follicles Dramatically Improves Their Developmental Competence1Biology of Reproduction, 2003
- Follicle stimulating hormone is required for ovarian follicle maturation but not male fertilityNature Genetics, 1997
- Expression of follicle stimulating hormone-receptor mRNA during gonadal developmentMolecular and Cellular Endocrinology, 1996
- Development in vitro of mouse oocytes from primordial folliclesBiology of Reproduction, 1996
- Pattern of Lactate Production and Steroidogenesis during Growth and Maturation of Mouse Ovarian Follicles in Vitro1Biology of Reproduction, 1993
- In Vitro DNA Synthesis by Isolated Preantral to Preovulatory Follicles from the Cyclic Mouse1Biology of Reproduction, 1991
- Serum suppresses the expression of hormonally induced functions in cultured granulosa cellsCell, 1980