Quantification of Lung Damage in an Elastase-Induced Mouse Model of Emphysema
Open Access
- 1 January 2012
- journal article
- Published by Hindawi Limited in International Journal of Biomedical Imaging
- Vol. 2012, 1-11
- https://doi.org/10.1155/2012/734734
Abstract
Objective. To define the sensitivity of microcomputed tomography- (micro-CT-) derived descriptors for the quantification of lung damage caused by elastase instillation.Materials and Methods. The lungs of 30 elastase treated and 30 control A/J mice were analyzed 1, 6, 12, and 24 hours and 7 and 17 days after elastase instillation using (i) breath-hold-gated micro-CT, (ii) pulmonary function tests (PFTs), (iii) RT-PCR for RNA cytokine expression, and (iv) histomorphometry. For the latter, an automatic, parallel software toolset was implemented that computes the airspace enlargement descriptors: mean linear interceptand weighted means of airspace diameters (,, and). A Support Vector Classifier was trained and tested based on three nonhistological descriptors usingas ground truth.Results.detected statistically significant differencesbetween the groups at all time points. Furthermore,at 1 hour (24 hours) was significantly lowerthanat 24 hours (7 days). The classifier trained on the micro-CT-derived descriptors achieves an area under the curve (AUC) of 0.95 well above the others (PFTS AUC = 0.71; cytokine AUC = 0.88).Conclusion. Micro-CT-derived descriptors are more sensitive than the other methods compared, to detectin vivoearly signs of the disease.
Keywords
Funding Information
- Ministerio de Sanidad, Servicios Sociales e Igualdad (PI070751, RTICC RD06/0020/0066, PSS 0100000-2008-2, MICINN PSE SINBAD, MCYT TEC2005-04732, MICINN DPI2009-14115-C03-03, MINECO DPI2012-38090-C03-02)
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