Optical Monitoring and Treatment of Potentially Lethal Wound Infections In Vivo
Open Access
- 1 June 2003
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 187 (11), 1717-1726
- https://doi.org/10.1086/375244
Abstract
We report on the use of optical techniques to monitor and treat Pseudomonas aeruginosa wound infections in mice. Bioluminescent bacteria transduced with a plasmid containing a bacterial lux gene operon allow the infection in excisional mouse wounds to be imaged by use of a sensitive charge-coupled device camera. Photodynamic therapy (PDT) targeted bacteria, by use of a polycationic photosensitizer conjugate, which is designed to penetrate the gram-negative cell wall and was topically applied to the wound and was followed by red-light illumination. There was a rapid light dose-dependent loss of luminescence, as measured by image analysis, in the wounds treated with conjugate and light, a loss that was not seen in untreated wounds, wounds treated with light alone, or wounds treated with conjugate alone. P. aeruginosa was invasive in our mouse model, and all 3 groups of control mice died within 5 days; in contrast, 90% of PDT-treated mice survived. PDT-treated wounds healed significantly faster than did silver nitrate-treated wounds, and this was not due to either inhibition of healing by silver nitrate or stimulation of healing by PDTKeywords
This publication has 29 references indexed in Scilit:
- PcrV Immunization Enhances Survival of Burned Pseudomonas aeruginosa -Infected MiceInfection and Immunity, 2001
- An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5alpha and EQ1Journal of Applied Microbiology, 2000
- Photodynamic antimicrobial chemotherapy (PACT)Journal of Antimicrobial Chemotherapy, 1998
- Secular Trends in Nosocomial Bloodstream Infections in a 55-Bed Cardiothoracic Intensive Care UnitThe Annals of Thoracic Surgery, 1998
- Simplified Agar Plate Method for Quantifying Viable BacteriaBioTechniques, 1997
- PULMONARY INFILTRATES IN THE CANCER PATIENT: New Approaches to an Old ProblemInfectious Disease Clinics of North America, 1996
- Photonic detection of bacterial pathogens in living hostsMolecular Microbiology, 1995
- Pseudomonas aeruginosa Bacteremia in Immunocompromised Children: Analysis of Factors Associated with a Poor OutcomeClinical Infectious Diseases, 1994
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samplesAnalytical Biochemistry, 1978
- Local and Systemic Therapy of Pseudomonas Septicemia in Burned MiceAnnals of Surgery, 1967