A rapid laser immunonephelometric assay for serum amyloid A (SAA) and its application to the diagnosis of kidney allograft rejection
- 1 April 1989
- journal article
- Published by Springer Science and Business Media LLC in Klinische Wochenschrift
- Vol. 67 (8), 447-451
- https://doi.org/10.1007/bf01725141
Abstract
We set up a laser nephelometric assay for the quantitation of serum amyloid A (SAA) in human plasma. Therefore monospecific antibodies were raised in sheep and used in parallel to measure SAA concentrations by nephelometry and also by radial immunodiffusion, an assay usually applied for determination of SAA. The nephelometric method is precise, simple and unlike radial immunodiffusion results are obtained within an hour. The antigen concentrations determined both by laser nephelometry and radial immunodiffusion correlated highly (r=0.98). As plasma SAA concentrations were reported to be a possible marker of kidney allograft rejection, the assay was applied to measure SAA concentrations in patients after kidney transplantation. Data were compared with clinical and other biochemical parameters. The period after kidney transplantation is reported for two cases, where SAA plasma concentrations were helpful in diagnosing allograft rejection. The rapid availability of the SAA plasma concentrations by nephelometry makes them a possible additional tool to decide quickly upon antirejection therapy.Keywords
This publication has 15 references indexed in Scilit:
- Standardisation of the quantitation of serum amyloid A protein (SAA) in human serumJournal of Immunological Methods, 1985
- CHANGES IN SERUM AMYLOID A PROTEIN AND BETA-2-MICROGLOBULIN IN ASSOCIATION WITH LIVER ALLOGRAFT REJECTIONTransplantation, 1984
- Measurement of serum amyloid A protein concentrations as test of renal allograft rejection in patients with initially non-functioning grafts.BMJ, 1984
- Quantitative radial immunodiffusion assay for serum amyloid A proteinJournal of Immunological Methods, 1983
- SERUM AMYLOID A PROTEINTransplantation, 1983
- Comparison of serum amyloid A protein and C-reactive protein concentrations in cancer and non-malignant disease.Journal of Clinical Pathology, 1983
- Preparative isoelectric focussing of apolipoproteins C and E from human very low density lipoproteinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
- SErum Amyloid a Protein in Amyloidosis, Rheumatic, and Neoplastic DiseasesArthritis & Rheumatism, 1979
- Isoelectric focusing in layers of granulated gels II. Preparative isoelectric focusingBiochimica et Biophysica Acta (BBA) - Protein Structure, 1975
- THE DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUMJCI Insight, 1955