Ultrafast chiral separations for high throughput enantiopurity analysis
- 18 November 2016
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 53 (3), 509-512
- https://doi.org/10.1039/c6cc08512a
Abstract
Recent developments in fast chromatographic enantioseparations now make high throughput analysis of enantiopurity on the order of a few seconds achievable. Nevertheless, routine chromatographic determinations of enantiopurity to support stereochemical investigations in pharmaceutical research and development, synthetic chemistry and bioanalysis are still typically performed on the 5–20 min timescale, with many practitioners believing that sub-minute enantioseparations are not representative of the molecules encountered in day to day research. In this study we develop ultrafast chromatographic enantioseparations for a variety of pharmaceutically-related drugs and intermediates, showing that sub-minute resolutions are now possible in the vast majority of cases by both supercritical fluid chromatography (SFC) and reversed phase liquid chromatography (RP-LC). Examples are provided illustrating how such methods can be routinely developed and used for ultrafast high throughput analysis to support enantioselective synthesis investigations.Keywords
This publication has 42 references indexed in Scilit:
- Salient Sub-Second SeparationsAnalytical Chemistry, 2016
- Instrumental Idiosyncrasies Affecting the Performance of Ultrafast Chiral and Achiral Sub/Supercritical Fluid ChromatographyAnalytical Chemistry, 2016
- Ultra-fast high-efficiency enantioseparations by means of a teicoplanin-based chiral stationary phase made on sub-2μm totally porous silica particles of narrow size distributionJournal of Chromatography A, 2016
- Pushing the speed limit in enantioselective supercritical fluid chromatographyJournal of Separation Science, 2015
- Gone in Seconds: Praxis, Performance, and Peculiarities of Ultrafast Chiral Liquid Chromatography with Superficially Porous ParticlesAnalytical Chemistry, 2015
- Estimating optimal time for fast chromatographic separationsJournal of Separation Science, 2014
- Support of academic synthetic chemistry using separation technologies from the pharmaceutical industryOrganic & Biomolecular Chemistry, 2014
- Processes To Separate EnantiomersAngewandte Chemie-International Edition, 2014
- Introducing Enantioselective Ultrahigh-Pressure Liquid Chromatography (eUHPLC): Theoretical Inspections and Ultrafast Separations on a New Sub-2-μm Whelk-O1 Stationary PhaseAnalytical Chemistry, 2012
- Chiral Separations: A Review of Current Topics and TrendsAnalytical Chemistry, 2011