Mapping Epitopes on a Protein Antigen by the Proteolysis of Antigen-Antibody Complexes
- 23 May 1986
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 232 (4753), 1001-1004
- https://doi.org/10.1126/science.2422757
Abstract
A monoclonal antibody bound to a protein antigen decreases the rate of proteolytic cleavage of the antigen, having the greatest effect on those regions involved in antibody contact. Thus, an epitope can be identified by the ability of the antibody to protect one region of the antigen more than others from proteolysis. By means of this approach, two distinct epitopes, both conformationally well-ordered, were characterized on horse cytochrome c.Keywords
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