Inhibitory Effects of Helianthus tuberosus Ethanol Extract on Dermatophagoides farina body-induced Atopic Dermatitis Mouse Model and Human Keratinocytes
Open Access
- 3 November 2018
- Vol. 10 (11), 1657
- https://doi.org/10.3390/nu10111657
Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by complex symptoms. To treat AD without adverse effects, alternative therapeutic agents are required. The tubers of Helianthus tuberosus L. (Jerusalem artichoke) have been used in folk remedies for diabetes and rheumatism. However, its effect on AD development remains unknown. Therefore, this study examined the inhibitory effect of H. tuberosus (HT) on AD skin symptoms using an NC/Nga mouse model and HaCaT keratinocytes. The effect of HT and associated molecular mechanisms were evaluated in Dermatophagoides farina body (Dfb)-induced AD mice and tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated HaCaT keratinocytes by ELISA, western blot, and histological analysis. Topical HT administration attenuated AD skin symptoms in Dfb-induced AD mice, with a significant reduction in the dermatitis score and production of inflammatory mediators. HT also decreased epidermal thickness and mast cell infiltration. Moreover, HT restored filaggrin expression and inhibited adhesion molecules in the mice. These effects were confirmed in vitro. Furthermore, HT suppressed the activation of NF-κB, Akt, and mitogen-activated protein kinase (MAPK) signaling pathways induced by TNF-α/IFN-γ. These results suggest that HT is a potential therapeutic agent or supplement for skin allergic inflammatory diseases such as AD.Keywords
Funding Information
- National Research Foundation of Korea (NRF) (NRF-2018R1C1B5041014)
This publication has 48 references indexed in Scilit:
- Cytokines and the Skin BarrierInternational Journal of Molecular Sciences, 2013
- Coal tar induces AHR-dependent skin barrier repair in atopic dermatitisJCI Insight, 2013
- The Role of Cytokines/Chemokines in the Pathogenesis of Atopic DermatitisCurrent problems in dermatology, 2011
- MAP17 is associated with the T-helper cell cytokine-induced down-regulation of filaggrin transcription in human keratinocytesExperimental Dermatology, 2010
- Cytokine modulation of atopic dermatitis filaggrin skin expressionJournal of Allergy and Clinical Immunology, 2009
- House Dust and Storage Mite Extracts Influence Skin Keratinocyte and Fibroblast FunctionInternational Archives of Allergy and Immunology, 2007
- Cytokines and chemokines orchestrate atopic skin inflammationJournal of Allergy and Clinical Immunology, 2006
- Signaling pathways regulating intercellular adhesion molecule 1 expression by endothelin 1: Comparison with interleukin‐1β in normal and scleroderma dermal fibroblastsArthritis & Rheumatism, 2006
- Akt mediates an angiogenic switch in transformed keratinocytesCarcinogenesis: Integrative Cancer Research, 2004
- Adhesion molecules in atopic dermatitis: VCAM-1 and ICAM-1 expression is increased in healthy-appearing skinAllergy, 1996