Lipopolysaccharide promotes the development of murine endometriosis‐like lesions via the nuclear factor‐kappa B pathway
- 31 January 2017
- journal article
- Published by Wiley in American Journal of Reproductive Immunology
- Vol. 77 (4)
- https://doi.org/10.1111/aji.12631
Abstract
Is lipopolysaccharide (LPS) involved in the development of endometriosis? BALB/c mice (n=69) were used for the murine endometriosis model. Mice with surgically induced endometriosis were injected with LPS intraperitoneally. After 4 weeks of LPS injections with or without the nuclear factor-kappa B (NF-κB) inhibitor, the extent of endometriosis-like lesions was evaluated. Expression of inflammatory factors in the implants was evaluated using real-time RT-PCR. Cell proliferation, angiogenic activity, inflammation, and NF-κB phosphorylation were assessed by immunohistochemical staining. Lipopolysaccharide increased total number, size, and mRNA expression of Ptgs-2, Vegf, Ccl-2, and Il-6 in endometriosis-like lesions. LPS also increased the percentage of Ki67-positive cells and enhanced the intensity and rate of positive cells of CD3, F4/80, and PECAM. Intense expression of phospho-NF-κB p65 after LPS administration was observed. Treatment with the NF-kB inhibitor negated these LPS-induced effects. LPS-induced pelvic inflammation status enhanced the development of murine endometriosis-like lesions via NF-κB pathway.Keywords
Funding Information
- JSPS KAKENHI (26462490, 15K10671)
This publication has 35 references indexed in Scilit:
- Nuclear factor–kappaB: a main regulator of inflammation and cell survival in endometriosis pathophysiologyFertility and Sterility, 2012
- Escherichia coli contamination of menstrual blood and effect of bacterial endotoxin on endometriosisFertility and Sterility, 2010
- Pyrrolidine dithiocarbamate inhibits nuclear factor- B pathway activation, and regulates adhesion, migration, invasion and apoptosis of endometriotic stromal cellsMolecular Human Reproduction, 2010
- Toll-Like Receptor 4 Mediates the Response of Epithelial and Stromal Cells to Lipopolysaccharide in the EndometriumPLOS ONE, 2010
- Lipopolysaccharide promoted proliferation and invasion of endometriotic stromal cells via induction of cyclooxygenase-2 expressionFertility and Sterility, 2010
- TAK1 activation for cytokine synthesis and proliferation of endometriotic cellsMolecular and Cellular Endocrinology, 2009
- Interleukin-10 attenuates TNF-α–induced interleukin-6 production in endometriotic stromal cellsFertility and Sterility, 2009
- ORIGINAL ARTICLE: TNFα Gene Silencing Reduced Lipopolysaccharide‐Promoted Proliferation of Endometriotic Stromal CellsAmerican Journal of Reproductive Immunology, 2009
- Lipopolysaccharide-promoted proliferation of endometriotic stromal cells via induction of tumor necrosis factor α and interleukin-8 expressionFertility and Sterility, 2004
- Endometriosis: its association with retrograde menstruation, dysmenorrhoea and tubal pathologyBJOG: An International Journal of Obstetrics and Gynaecology, 1986