Association of HPA axis-related genetic variation with stress reactivity and aggressive behaviour in pigs
Open Access
- 9 August 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Genomic Data
- Vol. 11 (1), 74
- https://doi.org/10.1186/1471-2156-11-74
Abstract
Background: Stress, elicited for example by aggressive interactions, has negative effects on various biological functions including immune defence, reproduction, growth, and, in livestock, on product quality. Stress response and aggressiveness are mutually interrelated and show large interindividual variation, partly attributable to genetic factors. In the pig little is known about the molecular-genetic background of the variation in stress responsiveness and aggressiveness. To identify candidate genes we analyzed association of DNA markers in each of ten genes (CRH g.233C>T, CRHR1 c.*866_867insA, CRHBP c.51G>A, POMC c.293_298del, MC2R c.306T>G, NR3C1 c.*2122A>G, AVP c.207A>G, AVPR1B c.1084A>G, UCN g.1329T>C, CRHR2 c.*13T>C) related to the hypothalamic-pituitary-adrenocortical (HPA) axis, one of the main stress-response systems, with various stress- and aggression-related parameters at slaughter. These parameters were: physiological measures of the stress response (plasma concentrations of cortisol, creatine kinase, glucose, and lactate), adrenal weight (which is a parameter reflecting activity of the central branch of the HPA axis over time) and aggressive behaviour (measured by means of lesion scoring) in the context of psychosocial stress of mixing individuals with different aggressive temperament. Results: The SNP NR3C1 c.*2122A>G showed association with cortisol concentration (p = 0.024), adrenal weight (p = 0.003) and aggressive behaviour (front lesion score, p = 0.012; total lesion score p = 0.045). The SNP AVPR1B c.1084A>G showed a highly significant association with aggressive behaviour (middle lesion score, p = 0.007; total lesion score p = 0.003). The SNP UCN g.1329T>C showed association with adrenal weight (p = 0.019) and aggressive behaviour (front lesion score, p = 0.029). The SNP CRH g.233C>T showed a significant association with glucose concentration (p = 0.002), and the polymorphisms POMC c.293_298del and MC2R c.306T>G with adrenal weight (p = 0.027 and p < 0.0001 respectively). Conclusions: The multiple and consistent associations shown by SNP in NR3C1 and AVPR1B provide convincing evidence for genuine effects of their DNA sequence variation on stress responsiveness and aggressive behaviour. Identification of the causal functional molecular polymorphisms would not only provide markers useful for pig breeding but also insight into the molecular bases of the stress response and aggressive behaviour in general.Keywords
This publication has 63 references indexed in Scilit:
- Human Glucocorticoid Receptor α Gene (NR3C1) Pharmacogenomics: Gene Resequencing and Functional GenomicsJournal of Clinical Endocrinology & Metabolism, 2009
- Psychobiology and molecular genetics of resilienceNature Reviews Neuroscience, 2009
- Animal evolution during domestication: the domesticated fox as a modelBioEssays, 2009
- Characterization of a Novel Gain of Function Glucocorticoid Receptor Knock-in MouseJournal of Biological Chemistry, 2009
- Vasopressin: Behavioral roles of an “original” neuropeptideProgress in Neurobiology, 2007
- Disruption of the vasopressin 1b receptor gene impairs the attack component of aggressive behavior in miceGenes, Brain and Behavior, 2006
- Functional Implication of an Arg307Gly Substitution in Corticosteroid-Binding Globulin, a Candidate Gene for a Quantitative Trait Locus Associated With Cortisol Variability and Obesity in PigGenetics, 2006
- Molecular characterization and evidencing of the porcine CRH gene as a functional-positional candidate for growth and body compositionBiochemical and Biophysical Research Communications, 2006
- Resident–Intruder Trait Aggression is Associated with Differences in Lysine Vasopressin and Serotonin Receptor 1A (5‐HT1A) mRNA Expression in the Brain of Pre‐Pubertal Female Domestic Pigs (Sus scrofa)Journal of Neuroendocrinology, 2005
- Statistical significance for genomewide studiesProceedings of the National Academy of Sciences of the United States of America, 2003