Osteonectin Influences Growth and Invasion of Pancreatic Cancer Cells
- 1 August 2005
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Annals of Surgery
- Vol. 242 (2), 224-234
- https://doi.org/10.1097/01.sla.0000171866.45848.68
Abstract
Objective: We sought to examine the expression and functional role of osteonectin in primary and metastatic pancreatic ductal adenocarcinoma (PDAC). Background: The glycoprotein osteonectin plays a vital role in cell–matrix interactions and is involved in various biologic processes. Overexpression of osteonectin is present in malignant tumors and correlates with disease progression and poor prognosis. Methods: Expression of osteonectin was analyzed by quantitative polymerase chain reaction and immunohistochemistry in pancreatic tissues and by enzyme-linked immunosorbent assay in the serum of patients and donors. Recombinant osteonectin and specific antisense oligonucleotides were used to examine the effects of osteonectin on induction of target genes, and on proliferation and invasiveness of pancreatic cancer cells. Results: There was a 31-fold increase in osteonectin mRNA levels in PDAC and a 16-fold increase in chronic pancreatitis as compared with the normal pancreas (P < 0.01). By immunohistochemistry, faint immunoreactivity was detected in the normal pancreas. In contrast, strong staining of the cancer cells was observed in addition to extensive osteonectin immunoreactivity in surrounding fibroblasts and in the extracellular matrix. In metastatic tissues, strong immunoreactivity was observed in fibroblasts and in extracellular matrix surrounding metastatic cancer cells, whereas the signal was absent in most tumor cells. In vitro studies showed that osteonectin was able to inhibit cancer cell growth while promoting invasiveness of pancreatic tumor cells. Conclusion: Osteonectin is markedly overexpressed in pancreatic cancer and has the potential to increase the invasiveness of pancreatic cancer cells.Keywords
This publication has 68 references indexed in Scilit:
- SPARC and tumor growth: Where the seed meets the soil?Journal of Cellular Biochemistry, 2004
- RUNX3 expression in primary and metastatic pancreatic cancerJournal of Clinical Pathology, 2004
- Expression and functional significance of CDC25B in human pancreatic ductal adenocarcinomaOncogene, 2004
- Cancer Statistics, 2004CA: A Cancer Journal for Clinicians, 2004
- Transcriptional upregulation of SPARC, in response to c-Jun overexpression, contributes to increased motility and invasion of MCF7 breast cancer cellsOncogene, 2002
- ANALYSIS OF THE GENE EXPRESSION OF SPARC AND ITS PROGNOSTIC VALUE FOR BLADDER CANCERJournal of Urology, 2001
- Induction of SPARC by VEGF in Human Vascular Endothelial CellsBiochemical and Biophysical Research Communications, 2001
- SPARC (Secreted Protein Acidic and Rich in Cysteine) Induces Apoptosis in Ovarian Cancer CellsThe American Journal of Pathology, 2001
- The Counteradhesive Protein SPARC Regulates an Endothelial Paracellular Pathway through Protein Tyrosine PhosphorylationBiochemical and Biophysical Research Communications, 1998
- High‐affinity and low‐affinity calcium binding and stability of the multidomain extracellular 40‐kDa basement membrane glycoprotein (BM‐40/SPARC/osteonectin)European Journal of Biochemistry, 1992