Analysis of immunoglobulin variable region genes from human IgG anti‐DNA hybridomas
- 1 July 1992
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 22 (7), 1719-1728
- https://doi.org/10.1002/eji.1830220709
Abstract
The molecular mechanisms leading to anti-double-stranded (ds)DNA antibody production in systemic lupus erythematosus (SLE) are poorly understood. We describe here the immunoglobulin variable region genes of six human hybridomas secreting IgG anti-dsDNA antibodies derived from three SLE patients. The monoclonal IgG anti-dsDNA antibodies have been shown to be of high affinity and no multireactivity was observed (Winkler et al., Clin. Exp. Immunol., 1991. 85: 379). The comparison of the variable region genes expressed in the hybridomas with known germ-line genes as well as with the germ-line counterparts from one patient shows that the VH and VL sequences are somatically mutated. The pattern and extent of the observed somatic mutations are suggestive for an antigen-driven selection of at least four of these B cell clones. Several VH and VL genes used by the hybridomas were found to be expressed in the natural antibody repertoire, in the restricted fetal repertoire and in B cell malignancies expressing the CD5 antigen.Keywords
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