Evaluation of a safe sputum processing method for detecting tuberculosis.
Open Access
- 1 May 1994
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 47 (5), 411-413
- https://doi.org/10.1136/jcp.47.5.411
Abstract
AIMS--To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS--A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS--Best results were obtained by auramine O staining after flotation. Staining by the Ziehl Neelsen method after flotation gave better results than direct Ziehl Neelsen staining without flotation. CONCLUSIONS--The flotation method with Ziehl Neelsen staining offers advantages for smear preparation in the tuberculosis control programmes of developing countries.Keywords
This publication has 11 references indexed in Scilit:
- Universal precautions--a critical review.1993
- Nosocomial Transmission of Multidrug-resistant Mycobacterium tuberculosisAnnals of Internal Medicine, 1992
- Multidrug-resistant TuberculosisAnnals of Internal Medicine, 1992
- An Outbreak of Tuberculosis Caused by Multiple-Drug-resistant Tubercle Bacilli among Patients with HIV InfectionAnnals of Internal Medicine, 1992
- Tuberculosis in Patients with Human Immunodeficiency Virus InfectionThe New England Journal of Medicine, 1991
- Mycobacterioses and the Acquired Immunodeficiency SyndromeAmerican Review of Respiratory Disease, 1987
- Laboratory-acquired tuberculosisTubercle, 1982
- The flotation method for detection of tubercle bacilli in sputum smearsTubercle, 1979
- Incidence of Tuberculosis Among Workers in Medical LaboratoriesBMJ, 1957
- Incidence of pulmonary tuberculosis among employees of tuberculosis hospitals.Published by Elsevier BV ,1952