Biochemical Characterization and Cellular Localization of 11S Type Storage Proteins in Olive (Olea europaea L.) Seeds
- 1 July 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (15), 5562-5570
- https://doi.org/10.1021/jf060203s
Abstract
9 páginas, 10 figuras, 2 tablas.The composition of seed storage proteins (SSPs) in olive endosperm and cotyledon has been analyzed. Precursor forms of these proteins are made up of individual proteins, which have been purified to homogeneity and further named p1−p5 (20.5, 21.5, 25.5, 27.5, and 30 kDa, respectively). N-terminal sequences of p1 and p2 proteins displayed relevant homology to the basic subunit of the 11S family of plant SSPs (legumins). Two-dimensional polyacrylamide gel electrophoresis experiments allowed us to verify the basic character of p1 and p2 and the acidic character of p3, p4, and p5 proteins. In addition, the putative presence of highly similar isoforms or posttranslational modifications of these polypeptides was detected. As a result, a model describing the putative association of p1−p5 proteins into subunits of α(acidic)/β(basic) type has been proposed. Solubility experiments have shown that the majority of these olive seed proteins from the 11S storage protein family are extracted with aqueous alcohol and only partially with water and diluted saline solutions, therefore suggesting their similarity to prolamines. Moreover, no visible differences were found in either subunit composition or 11S proteins mass among six olive cultivars examined. This result suggests that the synthesis of storage proteins is highly conserved in this plant species. By using a rabbit antiserum raised to p1 protein, the proteins have also been immunolocalized in olive seed tissues, showing that they accumulate in conspicuous protein bodies present in both the endosperm and the cotyledon.Peer revieweKeywords
This publication has 18 references indexed in Scilit:
- Molecular Analysis and Physicochemical Properties of Electrophoretic Variants of Wild Soybean Glycine soja Storage ProteinsJournal of Agricultural and Food Chemistry, 2005
- Chemical composition and nutrients availability for goats and sheep of two-stage olive cake and olive leavesAnimal Feed Science and Technology, 2003
- Role of Olive Seed in the Biogenesis of Virgin Olive Oil AromaJournal of Agricultural and Food Chemistry, 2003
- Storage and mobilization as antagonistic functional constraints on seed storage globulin evolutionJournal of Experimental Botany, 2003
- Protein Storage Bodies and VacuolesPlant Cell, 1999
- Subcellular localization of the 2S globulin narbonin in seeds of Vicia narbonensisPlanta, 1997
- Characterization of seed storage proteins in Populus and their homology with Populus vegetative storage proteinsTree Physiology, 1996
- Seed storage proteins in Fagaceae: similarity between Castanea globulins and Quercus glutelinsPlant Science, 1991
- Improved tools for biological sequence comparison.Proceedings of the National Academy of Sciences of the United States of America, 1988
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970