IgM lambda cytoplasmic crystals in three cases of immunocytoma: a clinical, cytochemical, and ultrastructural study.
Open Access
- 1 March 1978
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 31 (3), 250-258
- https://doi.org/10.1136/jcp.31.3.250
Abstract
Endoplasmic reticulum-associated crystals were seen in 1-10% of the bone-marrow lymphocytes and in the lymphocytes of the peripheral blood in three cases of immunocytoma. Their crystalline nature and their location in the cisternae of the rough endoplasmic reticulum was proved by ultrastructural study. IgM lambda in the crystals was demonstrated by fluorescent and peroxidase-labelled antibody methods. The crystals did not stain with the PAS reaction, suggesting that the immunoglobulin was not bound to a carbohydrate group. A defect in glucosyltransferase activity with failure to modify the immunoglobulins could explain the absence of the PAS reaction and the accumulation of immunoglobulin in crystalline before reaching the Golgi region.Keywords
This publication has 32 references indexed in Scilit:
- Stimulation by Propylthiouracil of the Hexose Monophosphate Shunt in Human Polymorphonuclear Leucocytes during PhagocytosisBritish Journal of Haematology, 1975
- Distinctive cytoplasmic inclusions in chronic lymphocytic leukaemiaEuropean Journal of Cancer (1965), 1975
- The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibodyJournal of Clinical Pathology, 1974
- Immunoglobulins on the Surface of Lymphoid Cells in Waldenström's MacroglobulinemiaJCI Insight, 1972
- Chronic Lymphocytosis with Lymphocyte InclusionsAnnals of Internal Medicine, 1972
- Immunocytoma o' mice an' men.BMJ, 1971
- IMMUNOGLOBULIN SYNTHESIS AND SECRETIONThe Journal of Experimental Medicine, 1971
- AN IMMUNOGLOBULIN-ENZYME BRIDGE METHOD FOR LOCALIZING TISSUE ANTIGENSJournal of Histochemistry & Cytochemistry, 1969
- CYTOCHEMISTRY AND ELECTRON MICROSCOPYThe Journal of cell biology, 1963
- IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODSThe Journal of cell biology, 1961