Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex
- 1 January 1994
- journal article
- Published by Informa UK Limited in Journal of Biomaterials Science, Polymer Edition
- Vol. 5 (4), 293-302
- https://doi.org/10.1163/156856294x00031
Abstract
We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.Keywords
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