A novel fluorescent biosensor for sequence-specific recognition of double-stranded DNA with the platform of graphene oxide
- 1 January 2011
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 136 (10), 2106-2110
- https://doi.org/10.1039/c1an15061h
Abstract
A fluorescent biosensor for sequence-specific recognition of double-stranded DNA (dsDNA) was developed based upon the DNA hybridization between dye-labeled single-stranded DNA (ssDNA) and double-stranded DNA. The fluorescence of FAM-labeled single-stranded DNA was quenched when it adsorbed on the surface of graphene oxide (GO). Upon addition of the target dsDNA, a homopyrimidine·homopurine part of dsDNA on the Simian virus 40 (SV40) (4424–4440, gp6), hybridization occurred between the dye-labeled DNA and the target dsDNA, which induced the dye-labeled DNA desorbed from the surface of GO, and turned on the fluorescence of the dye. Under the optimum conditions, the enhanced fluorescence intensity was proportional to the concentration of target dsDNA in the range 40.0–260 nM, and the detection limit was found to be 14.3 nM alongside the good sequence selectivity.Keywords
This publication has 29 references indexed in Scilit:
- A graphene-based fluorescent nanoprobe for silver(i) ions detection by using graphene oxide and a silver-specific oligonucleotideChemical Communications, 2010
- A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA AnalysisAdvanced Functional Materials, 2010
- Graphene Oxide-Facilitated Electron Transfer of Metalloproteins at Electrode SurfacesLangmuir, 2009
- Electrochemical Sensing and Biosensing Platform Based on Chemically Reduced Graphene OxideAnalytical Chemistry, 2009
- A Graphene Platform for Sensing BiomoleculesAngewandte Chemie, 2009
- Graphene-Based Single-Bacterium Resolution Biodevice and DNA Transistor: Interfacing Graphene Derivatives with Nanoscale and Microscale BiocomponentsNano Letters, 2008
- LNA functionalized gold nanoparticles as probes for double stranded DNA through triplex formationChemical Communications, 2008
- Processable aqueous dispersions of graphene nanosheetsNature Nanotechnology, 2008
- Preparation and characterization of graphene oxide paperNature, 2007
- Graphene-based composite materialsNature, 2006