Association between human oocyte developmental competence and expression levels of some cumulus genes
- 1 November 2007
- journal article
- Published by Bioscientifica in Reproduction
- Vol. 134 (5), 645-650
- https://doi.org/10.1530/rep-07-0182
Abstract
At present, oocyte selection is mainly based upon morphological criteria but it is generally acknowledged that its reliability requires further improvement. The aim of this study was to determine whether transcript levels in cumulus cells can provide a useful marker of oocyte developmental competence in vitro. A retrospective study was performed on cumulus cells isolated from 90 oocytes retrieved from 45 patients. Upon fertilization, 35 oocytes originated good-quality embryos and 36 developed into poor-quality embryos, whereas 19 failed to be fertilized. Semi-quantitative measurement of hyaluronic acid synthase 2 (HAS2), gremlin1 (GREM1), and pentraxin 3 (PTX3) mRNAs was performed and data for all genes were obtained from all the samples. Cumulus cells isolated from oocytes that originated high-quality embryos on day 3 of culture had HAS2 and GREM1 transcript levels higher than those detected in cells from oocytes that did not fertilize or developed into poor-quality embryos. No differences were observed in PTX3 levels. Results indicate that the measurement of HAS2 and GREM1 levels in cumulus cells would reliably complement the morphological evaluation providing a useful tool for selecting oocytes with greater chances to be fertilized and develop in vitro.Keywords
This publication has 21 references indexed in Scilit:
- Follicular Fuid Levels of the Long Pentraxin PTX3Reproductive Sciences, 2006
- The Art and Artifact of GDF9 Activity: Cumulus Expansion and the Cumulus Expansion-Enabling Factor1Biology of Reproduction, 2005
- Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte qualityFertility and Sterility, 2005
- Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVFHuman Reproduction, 2004
- Identification of viable embryos in IVF by non-invasive measurement of amino acid turnoverHuman Reproduction, 2004
- Growth Differentiation Factor 9 Regulates Expression of the Bone Morphogenetic Protein Antagonist GremlinOnline Journal of Public Health Informatics, 2004
- PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilizationDevelopment, 2004
- Knockout of Pentraxin 3, a Downstream Target of Growth Differentiation Factor-9, Causes Female SubfertilityMolecular Endocrinology, 2002
- Simple, sensitive and accurate method for the quantification of prothrombin mRNA by using competitive PCRBiochemical Journal, 2001
- Paracrine Actions Of Growth Differentiation Factor-9 in the Mammalian OvaryMolecular Endocrinology, 1999